Overview

  • Product name
  • Description
    Rabbit polyclonal to SP140
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SP140 aa 279-309 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q13342

  • Positive control
    • HeLa cells; Human kidney tissue; HL60 cell line lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab171141 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab171141 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 98 kDa.
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/10 - 1/50.

Target

  • Function
    Component of the nuclear body, also known as nuclear domain 10, PML oncogenic domain, and KR body. May be involved in the pathogenesis of acute promyelocytic leukemia and viral infection.
  • Tissue specificity
    High levels in spleen and peripheral blood leukocytes, much lower levels in thymus, prostate, ovary, small intestine, and colon. Very low levels in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas.
  • Sequence similarities
    Contains 1 bromo domain.
    Contains 1 HSR domain.
    Contains 1 PHD-type zinc finger.
    Contains 1 SAND domain.
  • Cellular localization
    Nucleus. Cytoplasm. Localized to nuclear structures termed LANDS, for LYSp100-associated nuclear domains. LANDS are globular, electron-dense structures most often found in the nucleoplasm, but also found at the nuclear membrane and in the cytoplasm, suggesting that these structures may traffic between the cytoplasm and the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • LY10_HUMAN antibody
    • Lymphoid restricted homolog of Sp100 antibody
    • Lymphoid specific SP100 homolog antibody
    • Lymphoid-restricted homolog of Sp100 antibody
    • LYSP100 A antibody
    • LYSp100 antibody
    • LYSP100 B antibody
    • LYSp100 protein antibody
    • LYSP100-A antibody
    • LYSP100-B antibody
    • LYSP100A antibody
    • LYSP100B antibody
    • MGC126440 antibody
    • Nuclear antigen Sp140 antibody
    • Nuclear autoantigen Sp-140 antibody
    • Nuclear autoantigen Sp140 antibody
    • Nuclear body protein SP140 antibody
    • SP140 antibody
    • SP140 nuclear body protein antibody
    • SP140 PEN antibody
    • Speckled 140 kDa antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling SP140 with ab171141 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Anti-SP140 antibody (ab171141) at 1/100 dilution + HL60 cell line lysate at 35 µg

    Predicted band size: 98 kDa

  • Confocal immunofluorescent analysis of Hela cells labeling SP140 with ab171141 at 1/25 dilution. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab171141  (1/25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1/400, 50 min at 37°C).  Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C).  Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).

References

ab171141 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab171141.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up