Anti-SP17 antibody (ab232826)

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Overview

  • Product name

    Anti-SP17 antibody
    See all SP17 primary antibodies

  • Description

    Rabbit polyclonal to SP17

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant full length protein (His-T7-tag) corresponding to Mouse SP17 aa 1-149. Expressed in E. coli. N-terminal tags.
    Sequence:

    MSIPFSNTHYRIPQGFGNLLEGLTREILREQPDNIPAFAAAYFENLLEKR EKTSFDPAEWGAKVEDRFYNNHAFKEQEQVEKCEQELAKSSGREETPVTP FEESTEEEREQEEAAALKIQSLFRGHVAREEVKKMKSDKNENLKEEADN


    Database link: Q62252

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Recombinant mouse SP17 protein; Mouse and rat testis tissue lysates. IHC-P: Mouse testis tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab232826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 17 kDa.
IHC-P Use a concentration of 5 - 20 µg/ml.

Target

  • Function

    Sperm surface zona pellucida binding protein. Helps to bind spermatozoa to the zona pellucida with high affinity. Might function in binding zona pellucida and carbohydrates.

  • Tissue specificity

    Testis and sperm specific.

  • Sequence similarities

    Contains 1 IQ domain.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • Band 34 antibody
      • Cancer/testis antigen 22 antibody
      • CT22 antibody
      • SP17 1 antibody
      • Sp17-1 antibody
      • SP17_HUMAN antibody
      • SPA17 antibody
      • Sperm autoantigenic protein 17 antibody
      • Sperm protein 17 antibody
      • Sperm surface protein Sp17 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP17 antibody (ab232826)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP17 antibody (ab232826)

      Formalin-fixed, paraffin-embedded mouse testis tissue stained for SP17 with ab232826 at 20 µg/ml in immunohistochemical analysis. DAB staining.

    • Western blot - Anti-SP17 antibody (ab232826)
      Western blot - Anti-SP17 antibody (ab232826)
      All lanes : Anti-SP17 antibody (ab232826) at 1 µg/ml

      Lane 1 : Mouse testis tissue lysate
      Lane 2 : Rat testis tissue lysate

      Secondary
      All lanes : HRP-linked Guinea pig anti-Rabbit at 1/2000 dilution

      Predicted band size: 17 kDa

    • Western blot - Anti-SP17 antibody (ab232826)
      Western blot - Anti-SP17 antibody (ab232826)
      Anti-SP17 antibody (ab232826) at 2 µg/ml + Recombinant mouse SP17 protein

      Predicted band size: 17 kDa

    References

    ab232826 has not yet been referenced specifically in any publications.

    Publishing research using ab232826? Please let us know so that we can cite the reference in this datasheet.

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