Overview

  • Product name

    Anti-SP2 transcription factor antibody
    See all SP2 transcription factor primary antibodies
  • Description

    Rabbit polyclonal to SP2 transcription factor
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human SP2 transcription factor (internal sequence). The exact sequence is proprietary.
    Database link: Q02086

  • Positive control

    • WB: Jurkat, Raji, NCI-H929 whole cell lysates; A431 and HepG2 whole cell extracts. IP: Raji whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab229468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 65 kDa.
IP 1/100 - 1/500.

Target

Images

  • All lanes : Anti-SP2 transcription factor antibody (ab229468) at 1/500 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
    Lane 3 : NCI-H929 (Human bone marrow lymphoblast cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 65 kDa



    7.5% SDS-PAGE gel.

  • All lanes : Anti-SP2 transcription factor antibody (ab229468) at 1/1000 dilution

    Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 65 kDa



    7.5% SDS-PAGE gel.

  • SP2 transcription factor was immunoprecipitated from Raji (human Burkitt's lymphoma cell line) whole cell extract with 3 µg ab229468. Western blot was performed from the immunoprecipitate using ab229468. Anti-Rabbit IgG was used as a secondary reagent.

    Lane 1: Raji whole cell extract.
    Lane 2: Control IgG IP in Raji whole cell extract.
    Lane 3: ab229468 IP in Raji whole cell extract.

References

ab229468 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - nuclear (preadipocyte model)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
30.7 µg
Specification
preadipocyte model
Blocking step
Milk as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 23°C

Dr. Sabriya Syed

Verified customer

Submitted May 16 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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