Anti-SP2 transcription factor antibody (ab229468)
Key features and details
- Rabbit polyclonal to SP2 transcription factor
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SP2 transcription factor antibody
See all SP2 transcription factor primary antibodies -
Description
Rabbit polyclonal to SP2 transcription factor -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Rhesus monkey
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Immunogen
Recombinant fragment within Human SP2 transcription factor (internal sequence). The exact sequence is proprietary.
Database link: Q02086 -
Positive control
- WB: Jurkat, Raji, NCI-H929 whole cell lysates; A431 and HepG2 whole cell extracts. IP: Raji whole cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab229468 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 65 kDa. | |
| IP | 1/100 - 1/500. |
Target
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Function
Binds to GC box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites. -
Sequence similarities
Belongs to the Sp1 C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 534403 Cow
- Entrez Gene: 6668 Human
- Entrez Gene: 78912 Mouse
- Entrez Gene: 303499 Rat
- Entrez Gene: 694834 Rhesus monkey
- Omim: 601801 Human
- SwissProt: Q5E9U0 Cow
- SwissProt: Q02086 Human
see all -
Alternative names
- Kiaa0048 antibody
- OTTHUMP00000196580 antibody
- SP2 antibody
see all
Images
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Western blot - Anti-SP2 transcription factor antibody (ab229468)All lanes : Anti-SP2 transcription factor antibody (ab229468) at 1/500 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : NCI-H929 (Human bone marrow lymphoblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 65 kDa7.5% SDS-PAGE gel.
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Western blot - Anti-SP2 transcription factor antibody (ab229468)All lanes : Anti-SP2 transcription factor antibody (ab229468) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 65 kDa7.5% SDS-PAGE gel.
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Immunoprecipitation - Anti-SP2 transcription factor antibody (ab229468)SP2 transcription factor was immunoprecipitated from Raji (human Burkitt's lymphoma cell line) whole cell extract with 3 µg ab229468. Western blot was performed from the immunoprecipitate using ab229468. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Raji whole cell extract.
Lane 2: Control IgG IP in Raji whole cell extract.
Lane 3: ab229468 IP in Raji whole cell extract.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab229468 has not yet been referenced specifically in any publications.
