Application
Western blot
Sample
Mouse Cell lysate - nuclear (preadipocyte model)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
30.7 µg
Specification
preadipocyte model
Blocking step
Milk as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBS, 0.1% Tween-20
Dilution
1/500
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: ECL anti-rabbit IgG HRP linked whole antibody
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 75 kDa
Dr. Sabriya Syed
Verified customer
Submitted May 16 2019