Overview

  • Product name

    Anti-SP3 antibody [EPR6806]
    See all SP3 primary antibodies
  • Description

    Rabbit monoclonal [EPR6806] to SP3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human SP3 aa 750-850. The exact sequence is proprietary.

  • Positive control

    • K562, HeLa, and 293T cell lysates; permeabilized HeLa cells.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 82 kDa).
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      Transcriptional factor that can act as an activator or repressor depending on isoform and/or post-translational modifications. Binds to GT and GC boxes promoter elements. Competes with SP1 for the GC-box promoters. Weak activator of transcription but can activate a number of genes involved in different processes such as cell-cycle regulation, hormone-induction and house-keeping.
    • Tissue specificity

      Ubiquitously expressed.
    • Sequence similarities

      Belongs to the Sp1 C2H2-type zinc-finger protein family.
      Contains 3 C2H2-type zinc fingers.
    • Post-translational
      modifications

      Not glycosylated.
      Acetylated by histone acetyltransferase p300, deacetylated by HDACs. Acetylation/deacetylation states regulate transcriptional activity. Acetylation appears to activate transcription. Alternate sumoylation and acetylation at Lys-551 also control transcriptional activity. Ceramides can also regulate acetylation/deacetylation events through altering the interaction of HDAC with SP3. In vitro, C(18)-ceramides, but not C(16)-ceramides, increase the interaction of HDAC1 with SP3 and enhance the deacetylation of SP3 and the subsequent repression of the TERT promoter.
      Sumoylated on all isoforms. Sumoylated on 2 sites in longer isoforms with Lys-551 being the major site. Sumoylation at this site promotes nuclear localization to the nuclear periphery, nuclear dots and PML nuclear bodies. Sumoylation on Lys-551 represses the transactivation activity, except for the largest isoform, L-Sp3, which has little effect on transactivation. Alternate sumoylation and acetylation at Lys-551 also control transcriptional activity.
    • Cellular localization

      Nucleus. Nucleus > PML body. Localizes to the nuclear periphery and in nuclear dots when sumoylated. Some localization in PML nuclear bodies.
    • Information by UniProt
    • Database links

    • Alternative names

      • D130027J01Rik antibody
      • DKFZp686O1631 antibody
      • GC binding transcription factor Sp 3 antibody
      • GC binding transcription factor Sp3 antibody
      • MGC105187 antibody
      • OTTMUSP00000014207 antibody
      • SP 3 antibody
      • Sp 3 transcription factor antibody
      • SP3 antibody
      • Sp3 transcription factor antibody
      • SP3_HUMAN antibody
      • Specificity protein 3 antibody
      • SPR 2 antibody
      • SPR-2 antibody
      • SPR2 antibody
      • Transcription factor SP 3 antibody
      • Transcription factor Sp3 antibody
      see all

    Images

    • All lanes : Anti-SP3 antibody [EPR6806] (ab129099) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 82 kDa

    • ab129099 at 1/100 dilution staining SP3 in permeabilized HeLa cells by Flow cytometry (red) or a rabbit IgG (negative) (green).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Chen J  et al. E2F1/SP3/STAT6 axis is required for IL-4-induced epithelial-mesenchymal transition of colorectal cancer cells. Int J Oncol 53:567-578 (2018). Read more (PubMed: 29901191) »
    • Kou XX  et al. Acetylated Sp1 inhibits PTEN expression through binding to PTEN core promoter and recruitment of HDAC1 and promotes cancer cell migration and invasion. Carcinogenesis 34:58-67 (2013). WB . Read more (PubMed: 23104175) »
    See all 4 Publications for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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