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Epigenetics and Nuclear Signaling Transcription Other factors
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RecombinantRabMAb

Recombinant Anti-SP3 antibody [EPR6806] (ab129099)

  • Datasheet
  • SDS
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Western blot - Anti-SP3 antibody [EPR6806] (ab129099)
  • Flow Cytometry - Anti-SP3 antibody [EPR6806] (ab129099)
  • OI-RD Scanning - Anti-SP3 antibody [EPR6806] (ab129099)
  • Anti-SP3 antibody [EPR6806] (ab129099)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6806] to SP3
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human

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Overview

  • Product name

    Anti-SP3 antibody [EPR6806]
    See all SP3 primary antibodies
  • Description

    Rabbit monoclonal [EPR6806] to SP3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human SP3 aa 750 to the C-terminus. The exact sequence is proprietary.

  • Positive control

    • K562, HeLa, and 293T cell lysates; permeabilized HeLa cells.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 4.14 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR6806
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors

Associated products

  • Alternative Versions

    • Anti-SP3 antibody [EPR6806] - BSA and Azide free (ab248298)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)
    • K-562 nuclear extract lysate (ab14851)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab129099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 82 kDa).
Flow Cyt
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Notes
WB
1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 82 kDa).
Flow Cyt
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      Transcriptional factor that can act as an activator or repressor depending on isoform and/or post-translational modifications. Binds to GT and GC boxes promoter elements. Competes with SP1 for the GC-box promoters. Weak activator of transcription but can activate a number of genes involved in different processes such as cell-cycle regulation, hormone-induction and house-keeping.
    • Tissue specificity

      Ubiquitously expressed.
    • Sequence similarities

      Belongs to the Sp1 C2H2-type zinc-finger protein family.
      Contains 3 C2H2-type zinc fingers.
    • Post-translational
      modifications

      Not glycosylated.
      Acetylated by histone acetyltransferase p300, deacetylated by HDACs. Acetylation/deacetylation states regulate transcriptional activity. Acetylation appears to activate transcription. Alternate sumoylation and acetylation at Lys-551 also control transcriptional activity. Ceramides can also regulate acetylation/deacetylation events through altering the interaction of HDAC with SP3. In vitro, C(18)-ceramides, but not C(16)-ceramides, increase the interaction of HDAC1 with SP3 and enhance the deacetylation of SP3 and the subsequent repression of the TERT promoter.
      Sumoylated on all isoforms. Sumoylated on 2 sites in longer isoforms with Lys-551 being the major site. Sumoylation at this site promotes nuclear localization to the nuclear periphery, nuclear dots and PML nuclear bodies. Sumoylation on Lys-551 represses the transactivation activity, except for the largest isoform, L-Sp3, which has little effect on transactivation. Alternate sumoylation and acetylation at Lys-551 also control transcriptional activity.
    • Cellular localization

      Nucleus. Nucleus > PML body. Localizes to the nuclear periphery and in nuclear dots when sumoylated. Some localization in PML nuclear bodies.
    • Target information above from: UniProt accession Q02447 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 6670 Human
      • Entrez Gene: 20687 Mouse
      • Omim: 601804 Human
      • SwissProt: Q02447 Human
      • SwissProt: O70494 Mouse
      • Unigene: 531587 Human
      • Unigene: 124328 Mouse
      • Unigene: 446209 Mouse
      • Unigene: 465887 Mouse
      see all
    • Alternative names

      • D130027J01Rik antibody
      • DKFZp686O1631 antibody
      • GC binding transcription factor Sp 3 antibody
      • GC binding transcription factor Sp3 antibody
      • MGC105187 antibody
      • OTTMUSP00000014207 antibody
      • SP 3 antibody
      • Sp 3 transcription factor antibody
      • SP3 antibody
      • Sp3 transcription factor antibody
      • SP3_HUMAN antibody
      • Specificity protein 3 antibody
      • SPR 2 antibody
      • SPR-2 antibody
      • SPR2 antibody
      • Transcription factor SP 3 antibody
      • Transcription factor Sp3 antibody
      see all

    Images

    • Western blot - Anti-SP3 antibody [EPR6806] (ab129099)
      Western blot - Anti-SP3 antibody [EPR6806] (ab129099)
      All lanes : Anti-SP3 antibody [EPR6806] (ab129099) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 82 kDa

    • Flow Cytometry - Anti-SP3 antibody [EPR6806] (ab129099)
      Flow Cytometry - Anti-SP3 antibody [EPR6806] (ab129099)
      ab129099 at 1/100 dilution staining SP3 in permeabilized HeLa cells by Flow cytometry (red) or a rabbit IgG (negative) (green).
    • OI-RD Scanning - Anti-SP3 antibody [EPR6806] (ab129099)
      OI-RD Scanning - Anti-SP3 antibody [EPR6806] (ab129099)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-SP3 antibody [EPR6806] (ab129099)
      Anti-SP3 antibody [EPR6806] (ab129099)

    Protocols

    • Western blot protocols
    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (4)

    Publishing research using ab129099? Please let us know so that we can cite the reference in this datasheet.

    ab129099 has been referenced in 4 publications.

    • Chen J  et al. E2F1/SP3/STAT6 axis is required for IL-4-induced epithelial-mesenchymal transition of colorectal cancer cells. Int J Oncol 53:567-578 (2018). PubMed: 29901191
    • Kou XX  et al. Acetylated Sp1 inhibits PTEN expression through binding to PTEN core promoter and recruitment of HDAC1 and promotes cancer cell migration and invasion. Carcinogenesis 34:58-67 (2013). WB . PubMed: 23104175
    • Bethune GC  et al. HER2 testing of multifocal invasive breast carcinoma: how many blocks are enough? Am J Clin Pathol 140:588-92 (2013). IHC ; Human . PubMed: 24045558
    • Robertson JF  et al. Activity of fulvestrant in HER2-overexpressing advanced breast cancer. Ann Oncol 21:1246-53 (2010). PubMed: 19875750

    Customer reviews and Q&As

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