Recombinant
Recombinant Anti-SP3 antibody [EPR6806] (ab129099)
- Datasheet
- References (4)
- Protocols
Overview
-
Product name
Anti-SP3 antibody [EPR6806]
See all SP3 primary antibodies -
Description
Rabbit monoclonal [EPR6806] to SP3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human SP3 aa 750-850. The exact sequence is proprietary.
-
Positive control
- K562, HeLa, and 293T cell lysates; permeabilized HeLa cells.
-
General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 4.14 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR6806 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab129099 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 82 kDa). | |
| Flow Cyt | 1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
Application notes
Is unsuitable for ICC/IF,IHC-P or IP.
Target
-
Function
Transcriptional factor that can act as an activator or repressor depending on isoform and/or post-translational modifications. Binds to GT and GC boxes promoter elements. Competes with SP1 for the GC-box promoters. Weak activator of transcription but can activate a number of genes involved in different processes such as cell-cycle regulation, hormone-induction and house-keeping. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the Sp1 C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers. -
Post-translational
modificationsNot glycosylated.
Acetylated by histone acetyltransferase p300, deacetylated by HDACs. Acetylation/deacetylation states regulate transcriptional activity. Acetylation appears to activate transcription. Alternate sumoylation and acetylation at Lys-551 also control transcriptional activity. Ceramides can also regulate acetylation/deacetylation events through altering the interaction of HDAC with SP3. In vitro, C(18)-ceramides, but not C(16)-ceramides, increase the interaction of HDAC1 with SP3 and enhance the deacetylation of SP3 and the subsequent repression of the TERT promoter.
Sumoylated on all isoforms. Sumoylated on 2 sites in longer isoforms with Lys-551 being the major site. Sumoylation at this site promotes nuclear localization to the nuclear periphery, nuclear dots and PML nuclear bodies. Sumoylation on Lys-551 represses the transactivation activity, except for the largest isoform, L-Sp3, which has little effect on transactivation. Alternate sumoylation and acetylation at Lys-551 also control transcriptional activity. -
Cellular localization
Nucleus. Nucleus > PML body. Localizes to the nuclear periphery and in nuclear dots when sumoylated. Some localization in PML nuclear bodies. - Information by UniProt
-
Database links
- Entrez Gene: 6670 Human
- Entrez Gene: 20687 Mouse
- Omim: 601804 Human
- SwissProt: Q02447 Human
- SwissProt: O70494 Mouse
- Unigene: 531587 Human
- Unigene: 124328 Mouse
- Unigene: 446209 Mouse
see all -
Alternative names
- D130027J01Rik antibody
- DKFZp686O1631 antibody
- GC binding transcription factor Sp 3 antibody
see all
Images
-
![Western blot - Anti-SP3 antibody [EPR6806] (ab129099)](https://www.abcam.com/ps/products/129/ab129099/Images/ab129099-143081-anti-sp3-antibody-epr6806-western-blot.jpg)
Western blot - Anti-SP3 antibody [EPR6806] (ab129099)All lanes : Anti-SP3 antibody [EPR6806] (ab129099) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 82 kDa -
![Flow Cytometry - Anti-SP3 antibody [EPR6806] (ab129099)](https://www.abcam.com/ps/products/129/ab129099/Images/ab129099-143080-anti-sp3-antibody-epr6806-flow-cytometry.jpg)
Flow Cytometry - Anti-SP3 antibody [EPR6806] (ab129099)ab129099 at 1/100 dilution staining SP3 in permeabilized HeLa cells by Flow cytometry (red) or a rabbit IgG (negative) (green). -
![Other - Anti-SP3 antibody [EPR6806] (ab129099)](https://www.abcam.com/ps/products/129/ab129099/Images/ab129099-143079-anti-sp3-antibody-epr6806.jpg)
Other - Anti-SP3 antibody [EPR6806] (ab129099)
Datasheets and documents
References
This product has been referenced in:
- Chen J et al. E2F1/SP3/STAT6 axis is required for IL-4-induced epithelial-mesenchymal transition of colorectal cancer cells. Int J Oncol 53:567-578 (2018). Read more (PubMed: 29901191) »
- Kou XX et al. Acetylated Sp1 inhibits PTEN expression through binding to PTEN core promoter and recruitment of HDAC1 and promotes cancer cell migration and invasion. Carcinogenesis 34:58-67 (2013). WB . Read more (PubMed: 23104175) »
