Overview

  • Product name

    Anti-SP3 antibody - N-terminal
    See all SP3 primary antibodies
  • Description

    Rabbit polyclonal to SP3 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human SP3 (N terminal). The exact sequence is proprietary.
    Database link: Q02447

  • Positive control

    • WB: HeLa cell extracts; Jurkat whole cell and nuclear extracts; Raji and NCI-H929 whole cell extracts. IP: Jurkat nuclear extract. ICC/IF: HeLa cells. IHC-P: Human breast carcinoma and ovarian carcinoma tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab227856 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 82 kDa.
IP 1/100 - 1/500.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Transcriptional factor that can act as an activator or repressor depending on isoform and/or post-translational modifications. Binds to GT and GC boxes promoter elements. Competes with SP1 for the GC-box promoters. Weak activator of transcription but can activate a number of genes involved in different processes such as cell-cycle regulation, hormone-induction and house-keeping.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the Sp1 C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • Post-translational
    modifications

    Not glycosylated.
    Acetylated by histone acetyltransferase p300, deacetylated by HDACs. Acetylation/deacetylation states regulate transcriptional activity. Acetylation appears to activate transcription. Alternate sumoylation and acetylation at Lys-551 also control transcriptional activity. Ceramides can also regulate acetylation/deacetylation events through altering the interaction of HDAC with SP3. In vitro, C(18)-ceramides, but not C(16)-ceramides, increase the interaction of HDAC1 with SP3 and enhance the deacetylation of SP3 and the subsequent repression of the TERT promoter.
    Sumoylated on all isoforms. Sumoylated on 2 sites in longer isoforms with Lys-551 being the major site. Sumoylation at this site promotes nuclear localization to the nuclear periphery, nuclear dots and PML nuclear bodies. Sumoylation on Lys-551 represses the transactivation activity, except for the largest isoform, L-Sp3, which has little effect on transactivation. Alternate sumoylation and acetylation at Lys-551 also control transcriptional activity.
  • Cellular localization

    Nucleus. Nucleus > PML body. Localizes to the nuclear periphery and in nuclear dots when sumoylated. Some localization in PML nuclear bodies.
  • Information by UniProt
  • Database links

  • Alternative names

    • D130027J01Rik antibody
    • DKFZp686O1631 antibody
    • GC binding transcription factor Sp 3 antibody
    • GC binding transcription factor Sp3 antibody
    • MGC105187 antibody
    • OTTMUSP00000014207 antibody
    • SP 3 antibody
    • Sp 3 transcription factor antibody
    • SP3 antibody
    • Sp3 transcription factor antibody
    • SP3_HUMAN antibody
    • Specificity protein 3 antibody
    • SPR 2 antibody
    • SPR-2 antibody
    • SPR2 antibody
    • Transcription factor SP 3 antibody
    • Transcription factor Sp3 antibody
    see all

Images

  • All lanes : Anti-SP3 antibody - N-terminal (ab227856) at 1/1000 dilution

    Lane 1 : WT HeLa cell extract
    Lane 2 : SP3 Knockout HeLa cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 82 kDa

  • All lanes : Anti-SP3 antibody - N-terminal (ab227856) at 1/1000 dilution

    Lane 1 : Wild-type HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
    Lane 2 : SP3 knockout HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 82 kDa



    7.5% SDS-PAGE gel.

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SP3 (green) using ab227856 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 5 minutes.

    Blue: Hoechst 33342 staining.

  • SP3 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract with 5 µg ab227856. Western blot was performed from the immunoprecipitate using ab227856. Anti-Rabbit IgG was used as a secondary reagent.

    Lane 1: Jurkat whole cell extract.

    Lane 2: Control IgG IP in Jurkat whole cell extract.

    Lane 3: ab227856 IP in Jurkat whole cell extract.

  • Paraffin-embedded human breast carcinoma tissue stained for SP3 using ab227856 at 1/1000 dilution in immunohistochemical analysis.

  • Paraffin-embedded human ovarian carcinoma tissue stained for SP3 using ab227856 at 1/1000 dilution in immunohistochemical analysis.

  • All lanes : Anti-SP3 antibody - N-terminal (ab227856) at 1/1000 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
    Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) nuclear extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 82 kDa



    7.5 % SDS-PAGE gel.

  • All lanes : Anti-SP3 antibody - N-terminal (ab227856) at 1/1000 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
    Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell extract
    Lane 3 : NCI-H929 whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 82 kDa



    7.5 % SDS-PAGE gel.

References

ab227856 has not yet been referenced specifically in any publications.

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