Overview

  • Product name

    Anti-Sp5 antibody [EPR11367(2)]
    See all Sp5 primary antibodies
  • Description

    Rabbit monoclonal [EPR11367(2)] to Sp5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Sp5 aa 100-200. The exact sequence is proprietary.
    Database link: Q6BEB4

  • Positive control

    • WB: HEK-293 transfected with a Sp5 construct whole cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab209385 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).

Target

  • Relevance

    Sp5 is a member of the Sp family of zinc finger transcription factors. Like other family members, the Sp5 protein contains a Cys2His2 zinc finger DNA binding domain at the C-terminus. Elevated expression of Sp5 has been noted in several human tumors including hepatocellular carcinoma, gastric cancer and colon cancer.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • Transcription factor Sp5 antibody

Images

  • All lanes : Anti-Sp5 antibody [EPR11367(2)] (ab209385) at 1/1000 dilution

    Lane 1 : Untransfected HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) transfected with Sp5 construct whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Overexpression lysates were kindly provided by Karl Willert, UCSD.

References

This product has been referenced in:

  • Huggins IJ  et al. The WNT target SP5 negatively regulates WNT transcriptional programs in human pluripotent stem cells. Nat Commun 8:1034 (2017). Read more (PubMed: 29044119) »
See 1 Publication for this product

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