Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11367(2)] to Sp5
- Suitable for: WB
- Reacts with: Human
Product nameAnti-Sp5 antibody [EPR11367(2)]
See all Sp5 primary antibodies
DescriptionRabbit monoclonal [EPR11367(2)] to Sp5
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Sp5 aa 100-200. The exact sequence is proprietary.
Database link: Q6BEB4
- WB: HEK-293 transfected with a Sp5 construct whole cell lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab209385 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).|
RelevanceSp5 is a member of the Sp family of zinc finger transcription factors. Like other family members, the Sp5 protein contains a Cys2His2 zinc finger DNA binding domain at the C-terminus. Elevated expression of Sp5 has been noted in several human tumors including hepatocellular carcinoma, gastric cancer and colon cancer.
- Transcription factor Sp5 antibody
All lanes : Anti-Sp5 antibody [EPR11367(2)] (ab209385) at 1/1000 dilution
Lane 1 : Untransfected HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) transfected with Sp5 construct whole cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 30 seconds
Blocking/Dilution buffer: 5% NFDM/TBST.
Overexpression lysates were kindly provided by Karl Willert, UCSD.
ab209385 has been referenced in 1 publication.
- Huggins IJ et al. The WNT target SP5 negatively regulates WNT transcriptional programs in human pluripotent stem cells. Nat Commun 8:1034 (2017). PubMed: 29044119