Overview

  • Product name

  • Description

    Rabbit polyclonal to SP6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human SP6 aa 177-254.
    Sequence:

    AGGQHLLGPPDGAKALEVAAPESQGLDSSLDGAARPKGSRRSVPRSSGQT VCRCPNCLEAERLGAPCGPDGGKKKHLH


    Database link: Q3SY56

  • Positive control

    • ICC/IF: Caco-2 cells. WB: NIH/3T3 and NBT-II cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab246952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.4 µg/ml.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100

Target

Images

  • PFA fixed, Triton X-100 permeabilized Caco-2 (Human colorectal adenocarcinoma cell line) cells labeling SP6 using ab246952 at 4 µg/ml (green) in ICC/IF.

  • All lanes : Anti-SP6 antibody (ab246952) at 0.4 µg/ml

    Lane 1 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
    Lane 2 : NBT-II (Rat cell line) cell lysate

References

ab246952 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab246952.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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