Anti-Sp7 / Osterix antibody (ab229258)
Key features and details
- Rabbit polyclonal to Sp7 / Osterix
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Sp7 / Osterix antibody
See all Sp7 / Osterix primary antibodies -
Description
Rabbit polyclonal to Sp7 / Osterix -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment within Human Sp7/ Osterix (internal sequence). The exact sequence is proprietary.
Database link: Q8TDD2 -
Positive control
- WB: U-2 OS nuclear extract.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229258 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 45 kDa.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 45 kDa. |
Target
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Function
Transcriptional activator essential for osteoblast differentiation. Binds to SP1 and EKLF consensus sequences and to other G/C-rich sequences. -
Tissue specificity
Restricted to bone-derived cell. -
Sequence similarities
Belongs to the Sp1 C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 531995 Cow
- Entrez Gene: 121340 Human
- Entrez Gene: 170574 Mouse
- Entrez Gene: 300260 Rat
- Omim: 606633 Human
- SwissProt: Q8TDD2 Human
- SwissProt: Q8VI67 Mouse
- Unigene: 209402 Human
see all -
Alternative names
- MGC126598 antibody
- Osterix antibody
- Osx antibody
see all
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab229258 has been referenced in 3 publications.
- Li S et al. Metallothionein 3 Promotes Osteoblast Differentiation in C2C12 Cells via Reduction of Oxidative Stress. Int J Mol Sci 22:N/A (2021). PubMed: 33919218
- Sun NY et al. Astragaloside-IV modulates NGF-induced osteoblast differentiation via the GSK3β/β-catenin signalling pathway. Mol Med Rep 23:N/A (2021). PubMed: 33179111
- Li X et al. Concentrated growth factor exudate enhances the proliferation of human periodontal ligament cells in the presence of TNF-a. Mol Med Rep 19:943-950 (2019). PubMed: 30535499