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    sp7--osterix-antibody-epr21034-ab209484.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Developmental Families Other
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RecombinantRabMAb

Recombinant Anti-Sp7 / Osterix antibody [EPR21034] (ab209484)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (6) Submit a question References (51)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody [EPR21034] (ab209484)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody [EPR21034] (ab209484)
  • Western blot - Anti-Sp7 / Osterix antibody [EPR21034] (ab209484)
  • Immunohistochemistry (Frozen sections) - Anti-Sp7 / Osterix antibody [EPR21034] (ab209484)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody [EPR21034] (ab209484)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody [EPR21034] (ab209484)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody [EPR21034] (ab209484)
  • Immunoprecipitation - Anti-Sp7 / Osterix antibody [EPR21034] (ab209484)
  • Anti-Sp7 / Osterix antibody [EPR21034] (ab209484)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21034] to Sp7 / Osterix
  • Suitable for: WB, IP, IHC-Fr, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Protein
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Recombinant Human Sp7 / Osterix protein (ab153589)
ELISA
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Mouse PAI1 ELISA Kit (SERPINE1) (ab197752)
Primary
Product image
Anti-Sp7 / Osterix antibody [EPR21034] - BSA and Azide free (ab227820)

View more associated products

Overview

  • Product name

    Anti-Sp7 / Osterix antibody [EPR21034]
    See all Sp7 / Osterix primary antibodies
  • Description

    Rabbit monoclonal [EPR21034] to Sp7 / Osterix
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Fr, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Saos-2 whole cell lysate. IHC-P: Mouse E15 embryo, rat E15 embryo, mouse E14.5 rib, rat E14.5 rib and human chondrosarcoma tissue. IHC-Fr: Mouse embryo E14.5 developing humerus tissue. IP: Saos-2 whole cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR21034
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • Other
    • Stem Cells
    • Mesenchymal Stem Cells
    • Intracellular
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Stem Cells
    • Mesenchymal Stem Cells
    • Osteogenesis
    • Developmental Biology
    • Organogenesis
    • Skeletal development
    • Bone

Associated products

  • Alternative Versions

    • Anti-Sp7 / Osterix antibody [EPR21034] - BSA and Azide free (ab227820)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Sp7 / Osterix protein (ab153589)
  • Related Buffer

    • Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)
  • Related Products

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab209484 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 45, 47 kDa (predicted molecular weight: 45 kDa).
IP
1/30.
IHC-Fr
1/500.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P (5)
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
1/1000. Detects a band of approximately 45, 47 kDa (predicted molecular weight: 45 kDa).
IP
1/30.
IHC-Fr
1/500.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Transcriptional activator essential for osteoblast differentiation. Binds to SP1 and EKLF consensus sequences and to other G/C-rich sequences.
  • Tissue specificity

    Restricted to bone-derived cell.
  • Sequence similarities

    Belongs to the Sp1 C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q8TDD2 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 121340 Human
    • Entrez Gene: 170574 Mouse
    • Entrez Gene: 300260 Rat
    • Omim: 606633 Human
    • SwissProt: Q8TDD2 Human
    • SwissProt: Q8VI67 Mouse
    • Unigene: 209402 Human
    • Unigene: 263284 Mouse
    • Alternative names

      • MGC126598 antibody
      • Osterix antibody
      • Osx antibody
      • Sp 7 antibody
      • SP7 antibody
      • Sp7 transcription factor antibody
      • SP7_HUMAN antibody
      • Transcription factor Sp7 antibody
      • Zinc finger protein osterix antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody [EPR21034] (ab209484)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody [EPR21034] (ab209484)

      IHC image of SP7/Osterix staining in a section of formalin-fixed paraffin-embedded normal rat E15 embryo performed on a Leica BOND® system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab22552, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody [EPR21034] (ab209484)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody [EPR21034] (ab209484)

      IHC image of SP7/Osterix staining in a section of formalin-fixed paraffin-embedded normal mouse E15 embryo performed on a Leica BOND® system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab22552, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Western blot - Anti-Sp7 / Osterix antibody [EPR21034] (ab209484)
      Western blot - Anti-Sp7 / Osterix antibody [EPR21034] (ab209484)
      Anti-Sp7 / Osterix antibody [EPR21034] (ab209484) at 1/1000 dilution + Saos-2 (human osteosarcoma cell line) whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 45 kDa
      Observed band size: 45, 47 kDa why is the actual band size different from the predicted?



      Blocking/Dilution buffer: 5% NFDM/TBST.

      Human Sp7 / Osterix has two isoforms (45 and 47 kDa). The 45kDa isoform is predominant (UniProt ID: Q8TDD2). In Saos-2 cells, we observed a faint, extra band at around 37kDa.

    • Immunohistochemistry (Frozen sections) - Anti-Sp7 / Osterix antibody [EPR21034] (ab209484)
      Immunohistochemistry (Frozen sections) - Anti-Sp7 / Osterix antibody [EPR21034] (ab209484)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse E14.5 developing humerus tissue labeling Sp7 / Osterix with ab209484 at 1/500 dilution, followed by AlexaFluor® 488 Goat anti-Rabbit (ab150077) at 1/1000 dilution. Positive staining on the mouse embryo E14.5 developing humerus (PMID:27134141) is observed. Counter stained with DAPI.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor® 488 Goat anti-Rabbit (ab150077) at 1/1000 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody [EPR21034] (ab209484)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody [EPR21034] (ab209484)

      Immunohistochemical analysis of paraffin-embedded rat E14.5 rib tissue labeling Sp7 / Osterix with ab209484 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on osteoblasts and chondrocytes of rat E14.5 rib (PMID: 17579353; PMID: 25977369) is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody [EPR21034] (ab209484)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody [EPR21034] (ab209484)

      Immunohistochemical analysis of paraffin-embedded mouse E14.5 rib tissue labeling Sp7 / Osterix with ab209484 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on osteoblasts and chondrocytes of mouse E14.5 rib (PMID: 17579353; PMID: 25977369) is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody [EPR21034] (ab209484)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody [EPR21034] (ab209484)

      Immunohistochemical analysis of paraffin-embedded human chondrosarcoma tissue labeling Sp7 / Osterix with ab209484 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on tumor cells of human chondrosarcoma is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-Sp7 / Osterix antibody [EPR21034] (ab209484)
      Immunoprecipitation - Anti-Sp7 / Osterix antibody [EPR21034] (ab209484)

      Sp7 / Osterix was immunoprecipitated from 0.35mg of Saos-2 (human osteosarcoma cell line) whole cell lysate with ab209484 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab209484 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

      Lane 1: Saos-2 whole cell lysate 10ug (Input). 

      Lane 2: ab209484 IP in Saos-2 whole cell lysate. 

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab190908 in Saos-2 whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 8 seconds.

    • Anti-Sp7 / Osterix antibody [EPR21034] (ab209484)
      Anti-Sp7 / Osterix antibody [EPR21034] (ab209484)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (51)

    Publishing research using ab209484? Please let us know so that we can cite the reference in this datasheet.

    ab209484 has been referenced in 51 publications.

    • Li N  et al. Altered BMP-Smad4 signaling causes complete cleft palate by disturbing osteogenesis in palatal mesenchyme. J Mol Histol 52:45-61 (2021). PubMed: 33159638
    • Matthews BG  et al. Heterogeneity of murine periosteum progenitors involved in fracture healing. Elife 10:N/A (2021). PubMed: 33560227
    • Zhang J  et al. Melatonin Promotes Heterotopic Ossification Through Regulation of Endothelial-Mesenchymal Transition in Injured Achilles Tendons in Rats. Front Cell Dev Biol 9:629274 (2021). PubMed: 33644068
    • Li R  et al. ETS Proto-Oncogene 1 Suppresses MicroRNA-128 Transcription to Promote Osteogenic Differentiation Through the HOXA13/ß-Catenin Axis. Front Physiol 12:626248 (2021). PubMed: 33746773
    • Huang Q  et al. 0.5-Gy X-ray irradiation induces reorganization of cytoskeleton and differentiation of osteoblasts. Mol Med Rep 23:N/A (2021). PubMed: 33760136
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

    Filter by Application

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    1-6 of 6 Abreviews

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Sp7 / Osterix antibody [EPR21034]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (Bone marrow)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH6
    Permeabilization
    No
    Specification
    Bone marrow
    Blocking step
    (agent) for 5 minute(s) · Concentration: 3%
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Frazer Bell

    Verified customer

    Submitted May 16 2022

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Sp7 / Osterix antibody [EPR21034]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Cat Tissue sections (Bone)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH6
    Permeabilization
    No
    Specification
    Bone
    Blocking step
    (agent) for 5 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Frazer Bell

    Verified customer

    Submitted May 16 2022

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Sp7 / Osterix antibody [EPR21034]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rabbit Cell (Mandible implant)
    Permeabilization
    Yes - Formalin circulation for 30 mins
    Specification
    Mandible implant
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Pamela Yelick

    Verified customer

    Submitted Aug 27 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Sp7 / Osterix antibody [EPR21034]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Osteoblasts)
    Antigen retrieval step
    None
    Permeabilization
    Yes - 20%DMSO
    Specification
    Osteoblasts
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Sep 30 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Sp7 / Osterix antibody [EPR21034]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Embryonic bone)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Embryonic bone
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Carl Hobbs

    Verified customer

    Submitted Feb 11 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Sp7 / Osterix antibody [EPR21034]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Embryo)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Embryo
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Carl Hobbs

    Verified customer

    Submitted Feb 11 2019

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