Overview

  • Product name

    Anti-Sp7 / Osterix antibody
    See all Sp7 / Osterix primary antibodies
  • Description

    Rabbit polyclonal to Sp7 / Osterix
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (ASSLLEEEVH YGSSPLAMLT AACSKFGGSS PLRDSTTLGK AGTKKPYSVG) of Human Sp7/ Osterix (NP_690599).

  • Positive control

    • Fetal lung lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab94744 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 45 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Images

  • Anti-Sp7 / Osterix antibody (ab94744) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 45 kDa



    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling Sp7 / Osterix with ab94744 at 5µg/ml.

References

This product has been referenced in:

  • Murahashi Y  et al. Multi-layered PLLA-nanosheets loaded with FGF-2 induce robust bone regeneration with controlled release in critical-sized mouse femoral defects. Acta Biomater 85:172-179 (2019). Read more (PubMed: 30583110) »
  • Shen C  et al. Comparison of osteogenic differentiation capacity in mesenchymal stem cells derived from human amniotic membrane (AM), umbilical cord (UC), chorionic membrane (CM), and decidua (DC). Cell Biosci 9:17 (2019). Read more (PubMed: 30792848) »
See all 23 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Lower Jaw)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10x Citrate Buffer pH6.0 (ab64214)
Permeabilization
No
Specification
Lower Jaw
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Paraformaldehyde

ウジャール バワール

Verified customer

Submitted Jun 13 2019

Application
Western blot
Sample
Human Cell lysate - whole cell (HUVECs)
Gel Running Conditions
Reduced Denaturing (9% SDS-PAGE)
Loading amount
20 µg
Treatment
10ng/ml IL-1 beta and 20uM Inhibitor for 15min
Specification
HUVECs
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted May 11 2016

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeLa cells)
Total protein in input
10 µg
Treatment
transfected flag-Osterix construct
Immuno-precipitation step
Protein A/G
Specification
HeLa cells

Natalia Artigas Robles

Verified customer

Submitted Apr 29 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue (femur)
Specification
femur

Abcam user community

Verified customer

Submitted Jan 06 2016

Answer

Thank you for contacting us.

Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products. However, I have passes on your details to our distributor (allied scientific). I am currently in contact with them to see whether it would be possible to give you a discount off ab94744 since your last antibody failed to work but unfortunately was no longer covered by our guarantee.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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