Product nameAnti-Sp7 / Osterix antibody - ChIP Grade
See all Sp7 / Osterix primary antibodies
DescriptionRabbit polyclonal to Sp7 / Osterix - ChIP Grade
Tested applicationsSuitable for: IHC-Fr, ChIP, ICC/IF, WB, IHC-P, IP, IHC-FoFrmore details
Species reactivityReacts with: Mouse, Rat, Human
- This antibody gave a positive signal for Immunohistochemistry (FFPE) in Rat bone.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
ChIP Related Products
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab22552 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration. PubMed: 20682789|
|ICC/IF||Use at an assay dependent concentration. PubMed: 20682789|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 46 kDa. PubMed: 17510056|
|IHC-P||1/100 - 1/500. Antigen retrieval is not essential but may optimise staining.|
|IP||Use at an assay dependent concentration.|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 20410296|
FunctionTranscriptional activator essential for osteoblast differentiation. Binds to SP1 and EKLF consensus sequences and to other G/C-rich sequences.
Tissue specificityRestricted to bone-derived cell.
Sequence similaritiesBelongs to the Sp1 C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers.
- Information by UniProt
- MGC126598 antibody
- Osterix antibody
- Osx antibody
All lanes : Anti-Sp7 / Osterix antibody - ChIP Grade (ab22552) at 1/500 dilution
Lane 1 : Protein Ladder
Lane 2 : SAOS cells at 20 µg with Milk, 2 hours, 21°C at 5 %
All lanes : Polyclonal Goat anti Rabbit at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 46 kDa
Exposure time: 5 minutes
ab22552 staining Osterix in rat femur sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was perfusion fixed with 4% PFA in 0.1M phosphate buffer (pH 7.4) via the left ventricle and decalcified in 10% EDTA. Samples were incubated with primary antibody (1/2000) for 12 hours at 4°C. Histofine simple stain rat MAX PO was used as the secondary antibody.
ab22552 staining Sp7/ Osterix in C2C12 cells by Immunocytochemistry/ Immunofluorescence.
Cells were stained with ab22552 at a 1/500 dilution followed by goat anti-rabbit IgG conjugated with Alexa Fluor 488 at a 1/400 dilution. Labeling was detected using a Leica TCS SL inverted laser scanning confocal microscope.
The subcellular localization of Osterx in C2C12 cells was analyzed by immunofluorescence with ab22552 in cells either transiently transfected with short Osterix construct or incubated in medium without serum. Scale bar 10 µm.
Immunohistochemical analysis of paraffin embedded mouse osteosarcoma tissue labeling Sp7/Osterix with ab22552 at 1/1000 (overnight at 4°C). Fixed 48h in 4% formol. Decalcified in 4% EDTA and 0.2% PFA pH7.4 before inclusion in paraffin. Biotin conjugated secondary 1h RT. Amplification StreptABC, substrate DAB.
ab22552 staining Osterix in Human bone marrow tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% NBF and blocked with Serum Free Protein Block (Dako) for 5 minutes at 24°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/1000) for 30 minutes at 24°C. An undiluted HRP-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.
Image courtesy of Human Protein Atlas
ab22552 staining Sp7 / osterix in human bone marrow tissue. Paraffin embedded human bone marrow tissue was incubated with ab22552 (1/25 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab22552 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
The image shows Osterix staining in rat bone tibiae using ab22552 at 1/100.
ab22552 staining Sp7 / Osterix in 21 days old rat developing long bone tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with methanol and blocking with 20% serum at 220C for 30 minutes was performed. The sample was incubated with primary antibody (1/100) for 3 hours at 220C. A Cy3®-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody at 1/1000 dilution.
This product has been referenced in:
- Mao Y et al. Tension force-induced bone formation in orthodontic tooth movement via modulation of the GSK-3ß/ß-catenin signaling pathway. J Mol Histol 49:75-84 (2018). IHC-P ; Rat . Read more (PubMed: 29224185) »
- Shao D et al. Effects of oral implants with miR-122-modified cell sheets on rat bone marrow mesenchymal stem cells. Mol Med Rep 17:1537-1544 (2018). WB ; Rat . Read more (PubMed: 29257226) »