Key features and details
- Rabbit polyclonal to Sp8
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-Sp8 antibody
DescriptionRabbit polyclonal to Sp8
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Human
Synthetic peptide corresponding to Mouse Sp8 aa 450 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in 46C whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab73494 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 48 kDa).|
FunctionTranscription factor which plays a key role in limb development. Positively regulates FGF8 expression in the apical ectodermal ridge (AER) and contributes to limb outgrowth in embryos.
Sequence similaritiesBelongs to the Sp1 C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers.
- Information by UniProt
- BTD antibody
- Buttonhead antibody
- Homolog of Btd antibody
Anti-Sp8 antibody (ab73494) at 1 µg/ml + 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205) at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?
Additional bands at: 23 kDa, 56 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
ab73494 detects a band at 46 kDa in 46c whole cell lysate. We are unsure as to why the band seen is slightly lower than predicted and would welcome any customer feedback and comments regarding this product and the data presented above.
ab73494 has been referenced in 1 publication.
- Borello U et al. SP8 Transcriptional Regulation of Cyclin D1 During Mouse Early Corticogenesis. Front Neurosci 12:119 (2018). ChIP-sequencing ; Mouse . PubMed: 29599703