Overview

  • Product name
  • Description
    Rabbit polyclonal to Sp8
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Mouse Sp8.

    (Peptide available as ab90836.)

  • Positive control
    • This antibody gave a positive signal in 46C whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab73494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 48 kDa).

Target

  • Function
    Transcription factor which plays a key role in limb development. Positively regulates FGF8 expression in the apical ectodermal ridge (AER) and contributes to limb outgrowth in embryos.
  • Sequence similarities
    Belongs to the Sp1 C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BTD antibody
    • Buttonhead antibody
    • Homolog of Btd antibody
    • OTTHUMP00000120017 antibody
    • Sp 8 antibody
    • SP8 antibody
    • Sp8 transcription factor antibody
    • SP8_HUMAN antibody
    • Specificity protein 8 antibody
    • Trans acting transcription factor 8 antibody
    • Transcription factor Sp8 antibody
    see all

Images

  • Anti-Sp8 antibody (ab73494) at 1 µg/ml + 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 48 kDa
    Observed band size: 46 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 23 kDa, 56 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes


    ab73494 detects a band at 46 kDa in 46c whole cell lysate. We are unsure as to why the band seen is slightly lower than predicted and would welcome any customer feedback and comments regarding this product and the data presented above.

References

This product has been referenced in:
  • Borello U  et al. SP8 Transcriptional Regulation of Cyclin D1 During Mouse Early Corticogenesis. Front Neurosci 12:119 (2018). CHIPseq ; Mouse . Read more (PubMed: 29599703) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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