Overview

  • Product name

    Anti-Sp8 antibody
  • Description

    Rabbit polyclonal to Sp8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide corresponding to Mouse Sp8 aa 450 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab90836)

  • Positive control

    • This antibody gave a positive signal in 46C whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab73494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 48 kDa).

Target

  • Function

    Transcription factor which plays a key role in limb development. Positively regulates FGF8 expression in the apical ectodermal ridge (AER) and contributes to limb outgrowth in embryos.
  • Sequence similarities

    Belongs to the Sp1 C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • BTD antibody
    • Buttonhead antibody
    • Homolog of Btd antibody
    • OTTHUMP00000120017 antibody
    • Sp 8 antibody
    • SP8 antibody
    • Sp8 transcription factor antibody
    • SP8_HUMAN antibody
    • Specificity protein 8 antibody
    • Trans acting transcription factor 8 antibody
    • Transcription factor Sp8 antibody
    see all

Images

  • Anti-Sp8 antibody (ab73494) at 1 µg/ml + 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 48 kDa
    Observed band size: 46 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 23 kDa, 56 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes


    ab73494 detects a band at 46 kDa in 46c whole cell lysate. We are unsure as to why the band seen is slightly lower than predicted and would welcome any customer feedback and comments regarding this product and the data presented above.

References

This product has been referenced in:

  • Borello U  et al. SP8 Transcriptional Regulation of Cyclin D1 During Mouse Early Corticogenesis. Front Neurosci 12:119 (2018). CHIPseq ; Mouse . Read more (PubMed: 29599703) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab73494.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up