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Rabbit polyclonal to Sp8
Predicted to work with:
Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Mouse Sp8.
(Peptide available as
This antibody gave a positive signal in 46C whole cell lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 48 kDa).
Transcription factor which plays a key role in limb development. Positively regulates FGF8 expression in the apical ectodermal ridge (AER) and contributes to limb outgrowth in embryos.
Belongs to the Sp1 C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers.
Information by UniProt
Homolog of Btd antibody
Western blot - Anti-Sp8 antibody (ab73494)
Anti-Sp8 antibody (ab73494) at 1 µg/ml + 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (
) at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
46 kDa (
why is the actual band size different from the predicted?
Additional bands at:
23 kDa, 56 kDa. We are unsure as to the identity of these extra bands.
ab73494 detects a band at 46 kDa in 46c whole cell lysate. We are unsure as to why the band seen is slightly lower than predicted and would welcome any customer feedback and comments regarding this product and the data presented above.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"