Overview

  • Product name

    Anti-Spa-1 antibody [331-9]
    See all Spa-1 primary antibodies
  • Description

    Mouse monoclonal [331-9] to Spa-1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human Spa-1 aa 1-1042.
    Database link: Q96FS4

  • Positive control

    • Human colon and tonsil tissues; Raji, HL60, Jurkat, HeLa and 293T cell lysates.
  • General notes

    Previously labelled as SIPA1. 

Properties

Applications

Our Abpromise guarantee covers the use of ab219085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 112 kDa.
IHC-P Use a concentration of 10 µg/ml.

Target

  • Function

    GTPase activator for the nuclear Ras-related regulatory proteins Rap1 and Rap2 in vitro, converting it to the putatively inactive GDP-bound state.
  • Tissue specificity

    Expressed in fetal as well as in adult tissues. Expressed abundantly in the lymphoid tissues such as thymus, spleen and peripheral blood lymphocytes and also shows a significant expression in the spinal cord.
  • Sequence similarities

    Contains 1 PDZ (DHR) domain.
    Contains 1 Rap-GAP domain.
  • Cellular localization

    Nucleus. Cytoplasm > perinuclear region. Endomembrane system. Mostly localized in the perinuclear membraneous region.
  • Information by UniProt
  • Database links

  • Alternative names

    • GTPase activating protein Spa 1 antibody
    • GTPase-activating protein Spa-1 antibody
    • MGC102688 antibody
    • MGC17037 antibody
    • p130 SPA-1 antibody
    • p130 SPA1 antibody
    • Signal induced proliferation associated 1 antibody
    • Signal induced proliferation associated gene 1 antibody
    • Signal induced proliferation associated protein 1 antibody
    • Signal-induced proliferation-associated protein 1 antibody
    • SIPA 1 antibody
    • Sipa-1 antibody
    • Sipa1 antibody
    • SIPA1_HUMAN antibody
    • Spa1 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon tissue labeling Spa-1 with ab219085 at 10 µg/ml.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling Spa-1 with ab219085 at 10 µg/ml.

  • All lanes : Anti-Spa-1 antibody [331-9] (ab219085) at 1 µg/ml

    Lane 1 : Raji cell lysate
    Lane 2 : HL60 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HeLa cell lysate
    Lane 5 : 293T cell lysate

    Predicted band size: 112 kDa

References

ab219085 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab219085.
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