Key features and details
- Mouse monoclonal [331-9] to Spa-1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Spa-1 antibody [331-9]
See all Spa-1 primary antibodies
DescriptionMouse monoclonal [331-9] to Spa-1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human Spa-1 aa 1-1042.
Database link: Q96FS4
- Human colon and tonsil tissues; Raji, HL60, Jurkat, HeLa and 293T cell lysates.
Previously labelled as SIPA1.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Constituents: 50% PBS, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab219085 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 112 kDa.|
|IHC-P||Use a concentration of 10 µg/ml.|
FunctionGTPase activator for the nuclear Ras-related regulatory proteins Rap1 and Rap2 in vitro, converting it to the putatively inactive GDP-bound state.
Tissue specificityExpressed in fetal as well as in adult tissues. Expressed abundantly in the lymphoid tissues such as thymus, spleen and peripheral blood lymphocytes and also shows a significant expression in the spinal cord.
Sequence similaritiesContains 1 PDZ (DHR) domain.
Contains 1 Rap-GAP domain.
Cellular localizationNucleus. Cytoplasm > perinuclear region. Endomembrane system. Mostly localized in the perinuclear membraneous region.
- Information by UniProt
- GTPase activating protein Spa 1 antibody
- GTPase-activating protein Spa-1 antibody
- MGC102688 antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon tissue labeling Spa-1 with ab219085 at 10 µg/ml.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling Spa-1 with ab219085 at 10 µg/ml.
All lanes : Anti-Spa-1 antibody [331-9] (ab219085) at 1 µg/ml
Lane 1 : Raji cell lysate
Lane 2 : HL60 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : 293T cell lysate
Predicted band size: 112 kDa
ab219085 has not yet been referenced specifically in any publications.