Overview

  • Product name
  • Description
    Rabbit polyclonal to Spa-1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Spa-1 aa 980-1042 (C terminal).
    Database link: Q96FS4

  • Positive control
    • 293T cell lysate, transfected with Spa-1; Mouse embryonic stem cells.
  • General notes

    Previously labelled as SIPA1. 

Properties

Applications

Our Abpromise guarantee covers the use of ab155024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 112 kDa.
ICC/IF 1/50 - 1/500.

Target

  • Function
    GTPase activator for the nuclear Ras-related regulatory proteins Rap1 and Rap2 in vitro, converting it to the putatively inactive GDP-bound state.
  • Tissue specificity
    Expressed in fetal as well as in adult tissues. Expressed abundantly in the lymphoid tissues such as thymus, spleen and peripheral blood lymphocytes and also shows a significant expression in the spinal cord.
  • Sequence similarities
    Contains 1 PDZ (DHR) domain.
    Contains 1 Rap-GAP domain.
  • Cellular localization
    Nucleus. Cytoplasm > perinuclear region. Endomembrane system. Mostly localized in the perinuclear membraneous region.
  • Information by UniProt
  • Database links
  • Alternative names
    • GTPase activating protein Spa 1 antibody
    • GTPase-activating protein Spa-1 antibody
    • MGC102688 antibody
    • MGC17037 antibody
    • p130 SPA-1 antibody
    • p130 SPA1 antibody
    • Signal induced proliferation associated 1 antibody
    • Signal induced proliferation associated gene 1 antibody
    • Signal induced proliferation associated protein 1 antibody
    • Signal-induced proliferation-associated protein 1 antibody
    • SIPA 1 antibody
    • Sipa-1 antibody
    • Sipa1 antibody
    • SIPA1_HUMAN antibody
    • Spa1 antibody
    see all

Images

  • All lanes : Anti-Spa-1 antibody (ab155024) at 1/500 dilution

    Lane 1 : 293T cell lysate, non-transfected
    Lane 2 : 293T cell lysate, transfected with Spa-1

    Predicted band size: 112 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of methanol-fixed Mouse embryonic stem cells labeling Spa-1 with ab155024 at 1/200. Lower image shows co-staining with Hoechst 33342.

References

ab155024 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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