Key features and details
- Rabbit polyclonal to Spa-1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Spa-1 antibody
See all Spa-1 primary antibodies
DescriptionRabbit polyclonal to Spa-1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chimpanzee, Rhesus monkey
Synthetic peptide corresponding to Human Spa-1 aa 992-1042.
Database link: NP_006738.3
- HeLa cells, 293T and mouse NIH3T3 whole cell lysates
Previously labelled as SIPA1.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab85928 was affinity purified using an epitope specific to Spa-1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab85928 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000. Predicted molecular weight: 112 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionGTPase activator for the nuclear Ras-related regulatory proteins Rap1 and Rap2 in vitro, converting it to the putatively inactive GDP-bound state.
Tissue specificityExpressed in fetal as well as in adult tissues. Expressed abundantly in the lymphoid tissues such as thymus, spleen and peripheral blood lymphocytes and also shows a significant expression in the spinal cord.
Sequence similaritiesContains 1 PDZ (DHR) domain.
Contains 1 Rap-GAP domain.
Cellular localizationNucleus. Cytoplasm > perinuclear region. Endomembrane system. Mostly localized in the perinuclear membraneous region.
- Information by UniProt
- GTPase activating protein Spa 1 antibody
- GTPase-activating protein Spa-1 antibody
- MGC102688 antibody
Detection of Spa-1 in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab85928 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot.
Detection: Chemiluminescence with an exposure time of 30 seconds.
All lanes : Anti-Spa-1 antibody (ab85928) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : mouse NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 112 kDa
Observed band size: 117 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
ab85928 has not yet been referenced specifically in any publications.