Recombinant Anti-Spa-1 antibody [EPR14134] (ab189929)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Spa-1 antibody [EPR14134]
See all Spa-1 primary antibodies -
Description
Rabbit monoclonal [EPR14134] to Spa-1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
within Human Spa-1 aa 950 to the C-terminus. The exact sequence is proprietary.
Database link: Q96FS4 -
Positive control
- Human fetal thymus, Raji, 293T and Raw 264.7 lysates; Human kidney tissue; A431 and 293T cells.
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General notes
Previously labelled as SIPA1.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14134 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab189929 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Detects a band of approximately 130 kDa (predicted molecular weight: 112 kDa). | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| ICC/IF | 1/250 - 1/500. | |
| Flow Cyt | 1/400. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
GTPase activator for the nuclear Ras-related regulatory proteins Rap1 and Rap2 in vitro, converting it to the putatively inactive GDP-bound state. -
Tissue specificity
Expressed in fetal as well as in adult tissues. Expressed abundantly in the lymphoid tissues such as thymus, spleen and peripheral blood lymphocytes and also shows a significant expression in the spinal cord. -
Sequence similarities
Contains 1 PDZ (DHR) domain.
Contains 1 Rap-GAP domain. -
Cellular localization
Nucleus. Cytoplasm > perinuclear region. Endomembrane system. Mostly localized in the perinuclear membraneous region. - Information by UniProt
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Database links
- Entrez Gene: 6494 Human
- Entrez Gene: 20469 Mouse
- Omim: 602180 Human
- SwissProt: Q96FS4 Human
- SwissProt: P46062 Mouse
- Unigene: 530477 Human
- Unigene: 3072 Mouse
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Alternative names
- GTPase activating protein Spa 1 antibody
- GTPase-activating protein Spa-1 antibody
- MGC102688 antibody
see all
Images
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![Western blot - Anti-Spa-1 antibody [EPR14134] (ab189929)](https://www.abcam.com/ps/products/189/ab189929/Images/ab189929-226401-wb.png)
Western blot - Anti-Spa-1 antibody [EPR14134] (ab189929)All lanes : Anti-Spa-1 antibody [EPR14134] (ab189929) at 1/5000 dilution
Lane 1 : Human fetal thymus
Lane 2 : Raji cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 112 kDa
Additional bands at: 130 kDa. We are unsure as to the identity of these extra bands. -
![Western blot - Anti-Spa-1 antibody [EPR14134] (ab189929)](https://www.abcam.com/ps/products/189/ab189929/Images/ab189929-226400-wb.png)
Western blot - Anti-Spa-1 antibody [EPR14134] (ab189929)Anti-Spa-1 antibody [EPR14134] (ab189929) at 1/1000 dilution + Raw264.7 at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 112 kDa
Additional bands at: 130 kDa. We are unsure as to the identity of these extra bands. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spa-1 antibody [EPR14134] (ab189929)](https://www.abcam.com/ps/products/189/ab189929/Images/ab189929-226399-ip.png)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spa-1 antibody [EPR14134] (ab189929)Immunohistochemical analysis of formalin fixed, paraffin embedded Human kidney tissue labeling Spa-1 with ab189929 at 1/50 dilution, and Prediluted HRP Polymer for Rabbit IgG as a secondary antibody. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Spa-1 antibody [EPR14134] (ab189929)](https://www.abcam.com/ps/products/189/ab189929/Images/ab189929-226398-ip.png)
Immunocytochemistry/ Immunofluorescence - Anti-Spa-1 antibody [EPR14134] (ab189929)Immunocytochemical analysis of A431 cells fixed in 4% paraformaldehyde labeling Spa-1 with ab189929 at 1/250 dilution, and Goat anti rabbit IgG(Alexa Fluor® 555) at 1/200 dilution as the secondary antibody. Counterstained with DAPI.
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![Flow Cytometry - Anti-Spa-1 antibody [EPR14134] (ab189929)](https://www.abcam.com/ps/products/189/ab189929/Images/ab189929-226397-flow.png)
Flow Cytometry - Anti-Spa-1 antibody [EPR14134] (ab189929)Flow cytometric analysis of 293T cells fixed in 2% paraformaldehyde labeling Spa-1 with ab189929 at 1/400 dilution, and Goat anti rabbit IgG (FITC) at 1/150 dilution as the secondary antibody.
Protocols
Datasheets and documents
References
ab189929 has not yet been referenced specifically in any publications.
