Overview

  • Product name
    Anti-Spa-1 antibody - N-terminal
    See all Spa-1 primary antibodies
  • Description
    Rabbit polyclonal to Spa-1 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Mouse Spa-1 (N terminal).
    Database link: P46062

  • Positive control
    • Human small intestine and tonsil tissues. NIH 3T3 lysate expressing recombinant SIPA1.
  • General notes

    Previously labelled as SIPA1. 

Properties

Applications

Our Abpromise guarantee covers the use of ab219772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000 - 1/5000. Detects a band of approximately 130 kDa (predicted molecular weight: 112 kDa).

ab219772 is capable of detecting both over-expressed and endogenous SIPA1.

Target

  • Function
    GTPase activator for the nuclear Ras-related regulatory proteins Rap1 and Rap2 in vitro, converting it to the putatively inactive GDP-bound state.
  • Tissue specificity
    Expressed in fetal as well as in adult tissues. Expressed abundantly in the lymphoid tissues such as thymus, spleen and peripheral blood lymphocytes and also shows a significant expression in the spinal cord.
  • Sequence similarities
    Contains 1 PDZ (DHR) domain.
    Contains 1 Rap-GAP domain.
  • Cellular localization
    Nucleus. Cytoplasm > perinuclear region. Endomembrane system. Mostly localized in the perinuclear membraneous region.
  • Information by UniProt
  • Database links
  • Alternative names
    • GTPase activating protein Spa 1 antibody
    • GTPase-activating protein Spa-1 antibody
    • MGC102688 antibody
    • MGC17037 antibody
    • p130 SPA-1 antibody
    • p130 SPA1 antibody
    • Signal induced proliferation associated 1 antibody
    • Signal induced proliferation associated gene 1 antibody
    • Signal induced proliferation associated protein 1 antibody
    • Signal-induced proliferation-associated protein 1 antibody
    • SIPA 1 antibody
    • Sipa-1 antibody
    • Sipa1 antibody
    • SIPA1_HUMAN antibody
    • Spa1 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human small intestine tissue labeling SIPA1 with ab219772 at 5 µg/ml.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling SIPA1 with ab219772 at 5 µg/ml.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling SIPA1 with ab219772 at 1.25 µg/ml.  The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. X40.

  • All lanes : Anti-Spa-1 antibody - N-terminal (ab219772) at 1/1250 dilution

    Lane 1 : SIPA1 over-expression lysate from Mouse NIH 3T3 cells
    Lane 2 : Lysate from NIH 3T3 cells mock-transfected with LacZGLB
    Lane 3 : SIPA1 over-expression lysate from Mouse NIH3T3 cells with immunizing peptide
    Lane 4 : Lysate from NIH 3T3 cells mock-transfected with LacZGLB with immunizing peptide

    Predicted band size: 112 kDa



    Lane 2 shows SIPA1 at a significantly reduced level compared to the transfected cells in Lane 1.

References

ab219772 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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