Product nameAnti-Spa-1 antibody - N-terminal
See all Spa-1 primary antibodies
DescriptionRabbit polyclonal to Spa-1 - N-terminal
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Mouse Spa-1 (N terminal).
Database link: P46062
- Human small intestine and tonsil tissues. NIH 3T3 lysate expressing recombinant SIPA1.
Previously labelled as SIPA1.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab219772 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/1000 - 1/5000. Detects a band of approximately 130 kDa (predicted molecular weight: 112 kDa).
ab219772 is capable of detecting both over-expressed and endogenous SIPA1.
FunctionGTPase activator for the nuclear Ras-related regulatory proteins Rap1 and Rap2 in vitro, converting it to the putatively inactive GDP-bound state.
Tissue specificityExpressed in fetal as well as in adult tissues. Expressed abundantly in the lymphoid tissues such as thymus, spleen and peripheral blood lymphocytes and also shows a significant expression in the spinal cord.
Sequence similaritiesContains 1 PDZ (DHR) domain.
Contains 1 Rap-GAP domain.
Cellular localizationNucleus. Cytoplasm > perinuclear region. Endomembrane system. Mostly localized in the perinuclear membraneous region.
- Information by UniProt
- GTPase activating protein Spa 1 antibody
- GTPase-activating protein Spa-1 antibody
- MGC102688 antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human small intestine tissue labeling SIPA1 with ab219772 at 5 µg/ml.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling SIPA1 with ab219772 at 5 µg/ml.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling SIPA1 with ab219772 at 1.25 µg/ml. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. X40.
All lanes : Anti-Spa-1 antibody - N-terminal (ab219772) at 1/1250 dilution
Lane 1 : SIPA1 over-expression lysate from Mouse NIH 3T3 cells
Lane 2 : Lysate from NIH 3T3 cells mock-transfected with LacZGLB
Lane 3 : SIPA1 over-expression lysate from Mouse NIH3T3 cells with immunizing peptide
Lane 4 : Lysate from NIH 3T3 cells mock-transfected with LacZGLB with immunizing peptide
Predicted band size: 112 kDa
Lane 2 shows SIPA1 at a significantly reduced level compared to the transfected cells in Lane 1.
ab219772 has not yet been referenced specifically in any publications.