Overview

  • Product name

    Anti-SPACA1 antibody - C-terminal
  • Description

    Rabbit polyclonal to SPACA1 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide corresponding to Mouse SPACA1 aa 287-305 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    QSPTDIPVHEDDALSEWNE


    Database link: Q9DA48

  • Positive control

    • Extracts from testis of wild type (+/+) and SPACA1 single (+/-) knock-out mice; mouse spermatids.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 50% Glycerol, 49% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191843 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/500.
WB 1/1000. Predicted molecular weight: 33 kDa.

Target

  • Function

    May be involved in sperm-egg fusion.
  • Tissue specificity

    Testis specific. At the equatorial segment and the inner acrosomal membrane of capacitated sperm.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • SACA1_HUMAN antibody
    • SAMP32 antibody
    • SPACA1 antibody
    • Sperm acrosomal membrane-associated protein 32 antibody
    • Sperm acrosome membrane-associated protein 1 antibody
    see all

Images

  • All lanes : Anti-SPACA1 antibody - C-terminal (ab191843) at 1/1000 dilution

    Lane 1 : Mouse testis tissue lysate
    Lane 2 : Mouse sperm cell lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 33 kDa



    Several proteins associated with sperm undergo modification during maturation. Multiple bands are reflection of post-translational modifications by glycosylation and phosphorylation.

  • All lanes : Anti-SPACA1 antibody - C-terminal (ab191843) at 1/1000 dilution

    Lane 1 : extracts from testis of wild type (+/+) mice
    Lane 2 : extracts from testis of SPACA1 single (+/-) knock-out mice
    Lane 3 : extracts from testis of SPACA1 double (-/-) knock-out mice

    Predicted band size: 33 kDa



    Basigin was used as a loading control.

  • Immunofluorescent analysis of round spermatids labeling SPACA1 with ab191843 at 1/100 and as a secondary antibody, Alexa-Fluor 488 conjugated anti-rabbit IgG antibody. Image at the right stained with Hoechst 33342.

References

ab191843 has not yet been referenced specifically in any publications.

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