Overview

  • Product name

    Anti-SPAG16 antibody
  • Description

    Goat polyclonal to SPAG16
  • Host species

    Goat
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human SPAG16 aa 217-228 (internal sequence) (Cysteine residue). (NP_078808.3)
    Sequence:

    (c)KLHYASYEPTIR


    Database link: Q8N0X2

  • Positive control

    • IHC-P: Human testis tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab240461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.

Steamed antigen retrieval with citrate buffer pH 6.

Target

  • Relevance

    Cilia and flagella are comprised of a microtubular backbone, the axoneme, which is organized by the basal body and surrounded by plasma membrane. SPAG16 encodes 2 major proteins that associate with the axoneme of sperm tail and the nucleus of postmeiotic germ cells, respectively.
  • Cellular localization

    Cytoskeleton; flagellum.
  • Database links

  • Alternative names

    • PF20 antibody
    • pf20 protein homolog antibody
    • sperm associated antigen 16 antibody
    • sperm associated WD repeat protein antibody
    • WD repeat domain 29 antibody
    • WRD29 antibody
    see all

Images

  • Paraffin-embedded human testis tissue stained for SPAG16 using ab240461 at 5 μg/ml in immunohistochemical analysis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

References

ab240461 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab240461.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up