Anti-SPAG6 antibody (ab155653)
Key features and details
- Rabbit polyclonal to SPAG6
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-SPAG6 antibody -
Description
Rabbit polyclonal to SPAG6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Xenopus laevis, Xenopus tropicalis -
Immunogen
Recombinant protein fragment, corresponding to a region within amino acids 180-465 of Human SPAG6 isoform 2 (UniProt ID: O75602).
-
Positive control
- NT2D1, IMR32, U87 MG and MCF7 cell lysates; Human MCF7 xenograft tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155653 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 50 kDa.
|
|
IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is also possible using Tris-EDTA buffer (pH 8.0).
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 50 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is also possible using Tris-EDTA buffer (pH 8.0). |
Target
-
Function
Important for structural integrity of the central apparatus in the sperm tail and for flagellar motility. -
Tissue specificity
Highly expressed in testis. -
Sequence similarities
Contains 8 ARM repeats. -
Cellular localization
Cytoplasm > cytoskeleton. Cell projection > cilium > flagellum. Associated with microtubules. Detected on the sperm flagellum. - Information by UniProt
-
Database links
- Entrez Gene: 539424 Cow
- Entrez Gene: 9576 Human
- Entrez Gene: 50525 Mouse
- Entrez Gene: 100286811 Pig
- Entrez Gene: 360747 Rat
- Entrez Gene: 734748 Xenopus laevis
- Entrez Gene: 548730 Xenopus tropicalis
- Omim: 605730 Human
see all -
Alternative names
- axoneme central apparatus protein antibody
- DKFZp434I153 antibody
- MGC26276 antibody
see all
Images
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (5)
ab155653 has been referenced in 5 publications.
- Bao Z et al. Aberrant expression of SPAG6 and NM23 predicts poor prognosis of human osteosarcoma. Front Genet 13:1012548 (2022). PubMed: 36199573
- Li X et al. Planar cell polarity defects and hearing loss in sperm-associated antigen 6 (Spag6)-deficient mice. Am J Physiol Cell Physiol 320:C132-C141 (2021). PubMed: 33175573
- Zhang R et al. SPAG6 promotes cell proliferation and inhibits apoptosis through the PTEN/PI3K/AKT pathway in Burkitt lymphoma. Oncol Rep 44:2021-2030 (2020). PubMed: 33000212
- Yin J et al. SPAG6 silencing induces apoptosis in the myelodysplastic syndrome cell line SKM-1 via the PTEN/PI3K/AKT signaling pathway in vitro and in vivo. Int J Oncol 53:297-306 (2018). PubMed: 29749435
- Li X et al. SPAG6 regulates cell apoptosis through the TRAIL signal pathway in myelodysplastic syndromes. Oncol Rep 37:2839-2846 (2017). PubMed: 28393201