Key features and details
- Rabbit polyclonal to SPAG6
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SPAG6 antibody
DescriptionRabbit polyclonal to SPAG6
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Xenopus laevis, Xenopus tropicalis
Recombinant protein fragment, corresponding to a region within amino acids 180-465 of Human SPAG6 isoform 2 (UniProt ID: O75602).
- NT2D1, IMR32, U87 MG and MCF7 cell lysates; Human MCF7 xenograft tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab155653 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 50 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is also possible using Tris-EDTA buffer (pH 8.0).|
FunctionImportant for structural integrity of the central apparatus in the sperm tail and for flagellar motility.
Tissue specificityHighly expressed in testis.
Sequence similaritiesContains 8 ARM repeats.
Cellular localizationCytoplasm > cytoskeleton. Cell projection > cilium > flagellum. Associated with microtubules. Detected on the sperm flagellum.
- Information by UniProt
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ab155653 has been referenced in 2 publications.
- Yin J et al. SPAG6 silencing induces apoptosis in the myelodysplastic syndrome cell line SKM-1 via the PTEN/PI3K/AKT signaling pathway in vitro and in vivo. Int J Oncol 53:297-306 (2018). PubMed: 29749435
- Li X et al. SPAG6 regulates cell apoptosis through the TRAIL signal pathway in myelodysplastic syndromes. Oncol Rep 37:2839-2846 (2017). PubMed: 28393201