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Rabbit polyclonal to SPAG6
HumanPredicted to work with:
Mouse, Rat, Cow, Pig, Xenopus laevis, Xenopus tropicalis
Recombinant protein fragment, corresponding to a region within amino acids 180-465 of Human SPAG6 isoform 2 (UniProt ID: O75602).
- NT2D1, IMR32, U87 MG and MCF7 cell lysates; Human MCF7 xenograft tissue.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/3000. Predicted molecular weight: 50 kDa.
||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is also possible using Tris-EDTA buffer (pH 8.0).
Important for structural integrity of the central apparatus in the sperm tail and for flagellar motility.
Highly expressed in testis.
Contains 8 ARM repeats.
Cytoplasm > cytoskeleton. Cell projection > cilium > flagellum. Associated with microtubules. Detected on the sperm flagellum.
Information by UniProt
- axoneme central apparatus protein antibody
- DKFZp434I153 antibody
- MGC26276 antibody
Western blot - Anti-SPAG6 antibody (ab155653)
Anti-SPAG6 antibody (ab155653) at 1/1000 dilution + MCF7 cell lysate at 30 µg
Predicted band size: 50 kDa
10% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPAG6 antibody (ab155653)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human MCF7 xenograft tissue, labeling SPAG6 using ab155653 at a 1/500 dilution.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"