Overview

  • Product name
  • Description
    Rabbit polyclonal to SPAG6
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig, Xenopus laevis, Xenopus tropicalis
  • Immunogen

    Recombinant protein fragment, corresponding to a region within amino acids 180-465 of Human SPAG6 isoform 2 (UniProt ID: O75602).

  • Positive control
    • NT2D1, IMR32, U87 MG and MCF7 cell lysates; Human MCF7 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab155653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 50 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is also possible using Tris-EDTA buffer (pH 8.0).

Target

Images

  • Anti-SPAG6 antibody (ab155653) at 1/1000 dilution + MCF7 cell lysate at 30 µg

    Predicted band size: 50 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human MCF7 xenograft tissue, labeling SPAG6 using ab155653 at a 1/500 dilution.

References

ab155653 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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