Anti-SPAG6 antibody (ab155653)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-SPAG6 antibody
See all SPAG6 primary antibodies -
DescriptionRabbit polyclonal to SPAG6
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-Pmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Xenopus laevis, Xenopus tropicalis
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Immunogen
Recombinant protein fragment, corresponding to a region within amino acids 180-465 of Human SPAG6 isoform 2 (UniProt ID: O75602).
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Positive control
- NT2D1, IMR32, U87 MG and MCF7 cell lysates; Human MCF7 xenograft tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab155653 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 50 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is also possible using Tris-EDTA buffer (pH 8.0). |
Target
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FunctionImportant for structural integrity of the central apparatus in the sperm tail and for flagellar motility.
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Tissue specificityHighly expressed in testis.
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Sequence similaritiesContains 8 ARM repeats.
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Cellular localizationCytoplasm > cytoskeleton. Cell projection > cilium > flagellum. Associated with microtubules. Detected on the sperm flagellum.
- Information by UniProt
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Database links
- Entrez Gene: 539424 Cow
- Entrez Gene: 9576 Human
- Entrez Gene: 50525 Mouse
- Entrez Gene: 100286811 Pig
- Entrez Gene: 360747 Rat
- Entrez Gene: 734748 Xenopus laevis
- Entrez Gene: 548730 Xenopus tropicalis
- Omim: 605730 Human
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Alternative names
- axoneme central apparatus protein antibody
- DKFZp434I153 antibody
- MGC26276 antibody
see all
Images
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Western blot - Anti-SPAG6 antibody (ab155653)Anti-SPAG6 antibody (ab155653) at 1/1000 dilution + MCF7 cell lysate at 30 µg
Predicted band size: 50 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPAG6 antibody (ab155653)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human MCF7 xenograft tissue, labeling SPAG6 using ab155653 at a 1/500 dilution.
References
ab155653 has not yet been referenced specifically in any publications.
