Anti-SPAG6 antibody (ab155653)
Key features and details
- Rabbit polyclonal to SPAG6
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SPAG6 antibody -
Description
Rabbit polyclonal to SPAG6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Xenopus laevis, Xenopus tropicalis
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Immunogen
Recombinant protein fragment, corresponding to a region within amino acids 180-465 of Human SPAG6 isoform 2 (UniProt ID: O75602).
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Positive control
- NT2D1, IMR32, U87 MG and MCF7 cell lysates; Human MCF7 xenograft tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab155653 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 50 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is also possible using Tris-EDTA buffer (pH 8.0). |
Target
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Function
Important for structural integrity of the central apparatus in the sperm tail and for flagellar motility. -
Tissue specificity
Highly expressed in testis. -
Sequence similarities
Contains 8 ARM repeats. -
Cellular localization
Cytoplasm > cytoskeleton. Cell projection > cilium > flagellum. Associated with microtubules. Detected on the sperm flagellum. - Information by UniProt
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Database links
- Entrez Gene: 539424 Cow
- Entrez Gene: 9576 Human
- Entrez Gene: 50525 Mouse
- Entrez Gene: 100286811 Pig
- Entrez Gene: 360747 Rat
- Entrez Gene: 734748 Xenopus laevis
- Entrez Gene: 548730 Xenopus tropicalis
- Omim: 605730 Human
see all -
Alternative names
- axoneme central apparatus protein antibody
- DKFZp434I153 antibody
- MGC26276 antibody
see all
Images
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Western blot - Anti-SPAG6 antibody (ab155653)Anti-SPAG6 antibody (ab155653) at 1/1000 dilution + MCF7 cell lysate at 30 µg
Predicted band size: 50 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPAG6 antibody (ab155653)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human MCF7 xenograft tissue, labeling SPAG6 using ab155653 at a 1/500 dilution.
Protocols
References (2)
ab155653 has been referenced in 2 publications.
- Yin J et al. SPAG6 silencing induces apoptosis in the myelodysplastic syndrome cell line SKM-1 via the PTEN/PI3K/AKT signaling pathway in vitro and in vivo. Int J Oncol 53:297-306 (2018). PubMed: 29749435
- Li X et al. SPAG6 regulates cell apoptosis through the TRAIL signal pathway in myelodysplastic syndromes. Oncol Rep 37:2839-2846 (2017). PubMed: 28393201
