Product nameAnti-SPAG7 antibody [EPR13391]
See all SPAG7 primary antibodies
DescriptionRabbit monoclonal [EPR13391] to SPAG7
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Species reactivityReacts with: Human
Recombinant fragment within Human SPAG7 aa 100 to the C-terminus. The exact sequence is proprietary.
Database link: O75391
- MCF7, SH-SY5Y and HeLa cell lysates; MCF7 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181187 in the following tested applications.
|WB||1/1000 - 1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).|
|ICC/IF||1/100 - 1/250.|
|IP||1/40 - 1/60.|
Tissue specificityDetected in fetal brain.
Sequence similaritiesContains 1 R3H domain.
- Information by UniProt
- ACRP antibody
- FSA 1 antibody
- FSA1 antibody
All lanes : Anti-SPAG7 antibody [EPR13391] (ab181187) at 1/5000 dilution
Lane 1 : MCF-7 cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/5000 dilution
Predicted band size: 26 kDa
Immunoprecipitation. ab181187 at 1/1000 labeling SPAG7 immunoprecipitated from HeLa cell lysate using ab181187 at 1/50.
Lane 2: Control IgG.
Immunofluorescent analysis of MCF7 cells labeling SPAG7 with ab181187 at 1/250 (green) with Goat anti rabbit IgG (Alexa Fluor®488) at 1/200 and DAPI staining (blue).
ab181187 has not yet been referenced specifically in any publications.