Overview

  • Product name

  • Description

    Goat polyclonal to SPAK
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Dog
  • Immunogen

    Synthetic peptide corresponding to Human SPAK aa 401-414 (internal sequence).
    Sequence:

    C-SQEKSRRVKEENPE


    Database link: NP_037365.2

  • Positive control

    • Human Brain (Cerebellum) lysates and in lysates of cell line Jurkat
  • General notes

     This product was previously labelled as STK39

     

Properties

Applications

Our Abpromise guarantee covers the use of ab106936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 59 kDa).
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    May act as a mediator of stress-activated signals.
  • Tissue specificity

    Predominantly expressed in brain and pancreas followed by heart, lung, kidney, skeletal muscle, liver, placenta and testis.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 protein kinase domain.
  • Domain

    PAPA box (proline-alanine repeats) may target the kinase to a specific subcellular location by facilitating interaction with intracellular proteins such as actin or actin-like proteins.
  • Cellular localization

    Cytoplasm. Nucleus. Nucleus when caspase-cleaved.
  • Information by UniProt
  • Database links

  • Alternative names

    • DCHT antibody
    • DKFZp686K05124 antibody
    • OTTHUMP00000165175 antibody
    • PASK antibody
    • Proline alanine rich STE20 related kinase antibody
    • Serine threonine kinase 39 (STE20/SPS1 homolog yeast) antibody
    • Serine threonine kinase 39 antibody
    • Serine/threonine protein kinase 39 antibody
    • Serine/threonine-protein kinase 39 antibody
    • Small intestine SPAK like kinase antibody
    • SPAK antibody
    • Ste 20 related kinase antibody
    • Ste-20-related kinase antibody
    • Ste20 like protein kinase antibody
    • STE20/SPS1 homolog antibody
    • STE20/SPS1 related proline alanine rich protein kinase antibody
    • STE20/SPS1-related proline-alanine-rich protein kinase antibody
    • STK 39 antibody
    • Stk39 antibody
    • STK39_HUMAN antibody
    see all

Images

  • All lanes : Anti-SPAK antibody (ab106936) at 1 µg/ml

    Lane 1 : mouse brain tissue lysate
    Lane 2 : rat brain tissue lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 59 kDa
    Observed band size: 65 kDa
    why is the actual band size different from the predicted?

  • Anti-SPAK antibody (ab106936) at 0.5 µg/ml + Jurkat lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 59 kDa
    Observed band size: 65 kDa why is the actual band size different from the predicted?

  • ab106936 (3.8µg/ml) staining of paraffin embedded Human Cerebral Cortex shows textured staining in the neurons. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

References

ab106936 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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