Overview

  • Product name

    Anti-SPAK (phospho S309) antibody
    See all SPAK primary antibodies
  • Description

    Rabbit polyclonal to SPAK (phospho S309)
  • Host species

    Rabbit
  • Specificity

    ab192798 detects endogenous levels of SPAK only when phosphorylated at Serine 311.

  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human SPAK (phospho S309) conjugated to keyhole limpet haemocyanin. Peptide sequence around phosphorylation site of Serine 311(S-F-R(p)-K-L).
    Database link: Q9UEW8

  • Positive control

    • COLO cell extract; Human brain tissue.
  • General notes

     This product was previously labelled as STK39

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 0.87% Sodium chloride, 50% Glycerol, 49% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab192798 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab192798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 59 kDa.
IHC-P 1/50 - 1/100.

Target

  • Function

    May act as a mediator of stress-activated signals.
  • Tissue specificity

    Predominantly expressed in brain and pancreas followed by heart, lung, kidney, skeletal muscle, liver, placenta and testis.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 protein kinase domain.
  • Domain

    PAPA box (proline-alanine repeats) may target the kinase to a specific subcellular location by facilitating interaction with intracellular proteins such as actin or actin-like proteins.
  • Cellular localization

    Cytoplasm. Nucleus. Nucleus when caspase-cleaved.
  • Information by UniProt
  • Database links

  • Alternative names

    • DCHT antibody
    • DKFZp686K05124 antibody
    • OTTHUMP00000165175 antibody
    • PASK antibody
    • Proline alanine rich STE20 related kinase antibody
    • Serine threonine kinase 39 (STE20/SPS1 homolog yeast) antibody
    • Serine threonine kinase 39 antibody
    • Serine/threonine protein kinase 39 antibody
    • Serine/threonine-protein kinase 39 antibody
    • Small intestine SPAK like kinase antibody
    • SPAK antibody
    • Ste 20 related kinase antibody
    • Ste-20-related kinase antibody
    • Ste20 like protein kinase antibody
    • STE20/SPS1 homolog antibody
    • STE20/SPS1 related proline alanine rich protein kinase antibody
    • STE20/SPS1-related proline-alanine-rich protein kinase antibody
    • STK 39 antibody
    • Stk39 antibody
    • STK39_HUMAN antibody
    see all

Images

  • All lanes : Anti-SPAK (phospho S309) antibody (ab192798) at 1/500 dilution

    Lane 1 : COLO cell extract
    Lane 2 : COLO cell extract with antigen-specific peptide

    Predicted band size: 59 kDa

  • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling SPAK (phospho S311) with ab192798 at 1/50 (left) or ab192798 preincubated with blocking peptide (right).

References

ab192798 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab192798.
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