Anti-SPAM1 antibody [1A4] (ab50694)
Key features and details
- Mouse monoclonal [1A4] to SPAM1
- Suitable for: ICC, WB
- Reacts with: Sheep
- Isotype: IgG1
Overview
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Product name
Anti-SPAM1 antibody [1A4]
See all SPAM1 primary antibodies -
Description
Mouse monoclonal [1A4] to SPAM1 -
Host species
Mouse -
Tested applications
Suitable for: ICC, WBmore details -
Species reactivity
Reacts with: Sheep
Does not react with: Rabbit, Cow, Pig -
Immunogen
Full length native protein (purified) corresponding to Sheep SPAM1.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
1A4 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab50694 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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Notes |
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ICC
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
Target
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Function
Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid. -
Tissue specificity
Testis. -
Sequence similarities
Belongs to the glycosyl hydrolase 56 family. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Alternative names
- epididymis secretory sperm binding protein Li 96n antibody
- HEL-S-96n antibody
- HYA1 antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab50694 has been referenced in 3 publications.
- Harrison RA Hyaluronidase in ram semen. Quantitative determination, and isolation of multiple forms. Biochem J 252:865-74 (1988). PubMed: 3421926
- Harrison RA & Gaunt SJ Multiple forms of ram and bull sperm hyaluronidase revealed by using monoclonal antibodies. J Reprod Fertil 82:777-85 (1988). PubMed: 3361511
- Harrison RA Preliminary characterization of the multiple forms of ram sperm hyaluronidase. Biochem J 252:875-82 (1988). PubMed: 3421927