Overview

  • Product name

    Anti-SPAM1 antibody [1D6]
    See all SPAM1 primary antibodies
  • Description

    Mouse monoclonal [1D6] to SPAM1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICCmore details
  • Species reactivity

    Reacts with: Sheep, Cow
    Does not react with: Rabbit, Pig
  • Immunogen

    Full length native protein (purified) corresponding to Sheep SPAM1.

  • General notes

     This product was previously labelled as Hyaluronidase PH20

     

Properties

Applications

Our Abpromise guarantee covers the use of ab50693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 57 kDa.
ICC Use at an assay dependent dilution.

Target

  • Function

    Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
  • Tissue specificity

    Testis.
  • Sequence similarities

    Belongs to the glycosyl hydrolase 56 family.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Alternative names

    • epididymis secretory sperm binding protein Li 96n antibody
    • HEL-S-96n antibody
    • HYA1 antibody
    • HYAL PH-20 antibody
    • HYAL PH20 antibody
    • Hyal-PH20 antibody
    • HYAL1 antibody
    • HYAL3 antibody
    • HYAL5 antibody
    • HYALP_HUMAN antibody
    • Hyaluronidase PH-20 antibody
    • Hyaluronoglucosaminidase PH-20 antibody
    • MGC26532 antibody
    • PH-20 Hyaluronidase antibody
    • PH20 antibody
    • PH20 Hyaluronidase antibody
    • SPAG15 antibody
    • SPAM-1 antibody
    • Spam1 antibody
    • sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) antibody
    • Sperm adhesion molecule 1 antibody
    • Sperm surface protein PH-20 antibody
    • Sperm surface protein PH20 antibody
    • zona pellucida binding antibody
    see all

References

This product has been referenced in:

  • Meuwese MC  et al. Endothelial surface layer degradation by chronic hyaluronidase infusion induces proteinuria in apolipoprotein E-deficient mice. PLoS One 5:e14262 (2010). Read more (PubMed: 21170388) »
  • Harrison RA & Gaunt SJ Multiple forms of ram and bull sperm hyaluronidase revealed by using monoclonal antibodies. J Reprod Fertil 82:777-85 (1988). Read more (PubMed: 3361511) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us.

The antibodies ab1828, ab23706 and ab50693 are tested with Bovine (Cow) tissue and are fully guaranteed so the Abreview discount is not valid for these.

I am happy to provide discount code for others antibodies however I will send 2 discount codes for a start, once customer gets good results then I will provide the codes for others. Could you please ask customer choosing the 2 antibodies from ab8448, ab49341, ab101892, ab101894 and ab125362.

Could you also provide customer's email and institution name?

Many thanks for your cooperation. I will look forward to hearing from you soon.

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Question
Answer

DISCOUNT CODE: *** Expiration date: April 5th, 2012 I am very pleased to hear you would like to accept our offer and test ab50693 in rat. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for rat and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.  

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