Anti-SPAM1 antibody (ab196596)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-SPAM1 antibody
See all SPAM1 primary antibodies -
Description
Rabbit polyclonal to SPAM1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human SPAM1 (internal sequence). The exact sequence is proprietary.
Database link: P38567 -
Positive control
- Hela cells, extracts of PC3, Raji, BT474 cells.
-
General notes
This product was previously labelled as Hyaluronidase PH20
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.87% Sodium chloride
without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab196596 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/50 - 1/200. | |
| IHC-P | 1/50 - 1/200. | |
| WB | 1/500 - 1/2000. Predicted molecular weight: 58 kDa. |
Target
-
Function
Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid. -
Tissue specificity
Testis. -
Sequence similarities
Belongs to the glycosyl hydrolase 56 family. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 6677 Human
- Omim: 600930 Human
- SwissProt: P38567 Human
- Unigene: 121494 Human
-
Alternative names
- epididymis secretory sperm binding protein Li 96n antibody
- HEL-S-96n antibody
- HYA1 antibody
see all
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-SPAM1 antibody (ab196596)Immunofluorescence analysis of Hela cells labelling SPAM1 using ab196596 at 1/50 (top) with DAPI blue for nuclear staining (bottom)
-
Western blot - Anti-SPAM1 antibody (ab196596)All lanes : Anti-SPAM1 antibody (ab196596) at 1/500 dilution
Lane 1 : PC3 cell extract
Lane 2 : Raji cell extract
Lane 3 : BT474 cell extract
Predicted band size: 58 kDa
Protocols
Datasheets and documents
References
ab196596 has not yet been referenced specifically in any publications.
