Key features and details
- Rabbit polyclonal to SPAM1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SPAM1 antibody
See all SPAM1 primary antibodies
DescriptionRabbit polyclonal to SPAM1
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Recombinant fragment within Human SPAM1 (internal sequence). The exact sequence is proprietary.
Database link: P38567
- Hela cells, extracts of PC3, Raji, BT474 cells.
This product was previously labelled as Hyaluronidase PH20
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.87% Sodium chloride
without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab196596 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 58 kDa.|
FunctionInvolved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
Sequence similaritiesBelongs to the glycosyl hydrolase 56 family.
Cellular localizationCell membrane.
- Information by UniProt
- epididymis secretory sperm binding protein Li 96n antibody
- HEL-S-96n antibody
- HYA1 antibody
ab196596 has been referenced in 1 publication.
- Hong Y et al. Degradation of tumour stromal hyaluronan by small extracellular vesicle-PH20 stimulates CD103+ dendritic cells and in combination with PD-L1 blockade boosts anti-tumour immunity. J Extracell Vesicles 8:1670893 (2019). PubMed: 31632619