Anti-SPAM1 antibody (ab196596)

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Key features and details

  • Rabbit polyclonal to SPAM1
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-SPAM1 antibody
    See all SPAM1 primary antibodies

  • Description

    Rabbit polyclonal to SPAM1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human SPAM1 (internal sequence). The exact sequence is proprietary.
    Database link: P38567

  • Positive control

    • Hela cells, extracts of PC3, Raji, BT474 cells.

  • General notes

     This product was previously labelled as Hyaluronidase PH20

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab196596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/200.
WB 1/500 - 1/2000. Predicted molecular weight: 58 kDa.

Target

  • Function

    Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.

  • Tissue specificity

    Testis.

  • Sequence similarities

    Belongs to the glycosyl hydrolase 56 family.

  • Post-translational
    modifications

    N-glycosylated.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • epididymis secretory sperm binding protein Li 96n antibody
      • HEL-S-96n antibody
      • HYA1 antibody
      • HYAL PH-20 antibody
      • HYAL PH20 antibody
      • Hyal-PH20 antibody
      • HYAL1 antibody
      • HYAL3 antibody
      • HYAL5 antibody
      • HYALP_HUMAN antibody
      • Hyaluronidase PH-20 antibody
      • Hyaluronoglucosaminidase PH-20 antibody
      • MGC26532 antibody
      • PH-20 Hyaluronidase antibody
      • PH20 antibody
      • PH20 Hyaluronidase antibody
      • SPAG15 antibody
      • SPAM-1 antibody
      • Spam1 antibody
      • sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) antibody
      • Sperm adhesion molecule 1 antibody
      • Sperm surface protein PH-20 antibody
      • Sperm surface protein PH20 antibody
      • zona pellucida binding antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-SPAM1 antibody (ab196596)
      Immunocytochemistry/ Immunofluorescence - Anti-SPAM1 antibody (ab196596)

      Immunofluorescence analysis of Hela cells labelling SPAM1 using ab196596 at 1/50 (top) with DAPI blue for nuclear staining (bottom)

    • Western blot - Anti-SPAM1 antibody (ab196596)
      Western blot - Anti-SPAM1 antibody (ab196596)
      All lanes : Anti-SPAM1 antibody (ab196596) at 1/500 dilution

      Lane 1 : PC3 cell extract
      Lane 2 : Raji cell extract
      Lane 3 : BT474 cell extract

      Predicted band size: 58 kDa

    References (1)

    Publishing research using ab196596? Please let us know so that we can cite the reference in this datasheet.

    ab196596 has been referenced in 1 publication.

    • Hong Y  et al. Degradation of tumour stromal hyaluronan by small extracellular vesicle-PH20 stimulates CD103+ dendritic cells and in combination with PD-L1 blockade boosts anti-tumour immunity. J Extracell Vesicles 8:1670893 (2019). PubMed: 31632619

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