Anti-SPANX antibody (ab47252)
Key features and details
- Rabbit polyclonal to SPANX
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SPANX antibody
See all SPANX primary antibodies -
Description
Rabbit polyclonal to SPANX -
Host species
Rabbit -
Specificity
The immunogen sequence is present within SPANX-A, SPANX-B, SPANX-C, SPANX-D and SPANX-N, but not present within SPANX-E. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla -
Immunogen
Synthetic peptide:
GDSDPQPAPKKMKTSES
, corresponding to internal sequence amino acids 31-47 of Human SPANX C -
Positive control
- Nuclear extract from VWM105 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab47252 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 11 kDa.
|
Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 11 kDa. |
Target
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Tissue specificity
Detected in testis, melanoma and bladder carcinoma. -
Sequence similarities
Belongs to the SPAN-X family. -
Developmental stage
Detected in round and elongating spermatids. -
Cellular localization
Cytoplasm. Nucleus. Associated with nuclear craters. - Information by UniProt
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Database links
- Entrez Gene: 64663 Human
- Omim: 300330 Human
- SwissProt: A0FGV1 Human
- SwissProt: A0FGV4 Human
- SwissProt: A0FGW6 Human
- SwissProt: A0FGX0 Human
- SwissProt: Q32WM2 Human
- SwissProt: Q9NY87 Human
see all -
Alternative names
- Cancer-testis-associated protein CTp11 antibody
- Cancer/testis antigen 11.3 antibody
- Cancer/testis-associated protein CTp11 antibody
see all
Images
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All lanes : Anti-SPANX antibody (ab47252) at 1/1000 dilution
Lane 1 : Nuclear extract from VWM105 cells
Lane 2 : purified recombinant
SPANX-C fusion protein.
Lane 3 : purified
recombinant SPANX-B, protein.
Lane 4 : purified
recombinant SPANX-C, protein.
Lane 5 : purified
recombinant SPANX-N, protein.
Secondary
Lanes 1-2 : IRDye™800 conjugated Gt-a-
Rabbit IgG [H&L] MX (611-132-122)
Predicted band size: 11 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa, 70 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Datasheets and documents
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Datasheet download
References (1)
ab47252 has been referenced in 1 publication.
- Lazar I et al. SPANX Control of Lamin A/C Modulates Nuclear Architecture and Promotes Melanoma Growth. Mol Cancer Res 18:1560-1573 (2020). PubMed: 32571981