Key features and details
- Rabbit polyclonal to SPANX
- Suitable for: ELISA, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SPANX antibody
See all SPANX primary antibodies
DescriptionRabbit polyclonal to SPANX
SpecificityThe immunogen sequence is present within SPANX-A, SPANX-B, SPANX-C, SPANX-D and SPANX-N, but not present within SPANX-E.
Tested applicationsSuitable for: ELISA, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla
- Nuclear extract from VWM105 cells
General notesDilute only prior to immediate use.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab47252 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IF (microscopy): Use at an assay dependent dilution.
WB: 1/500 - 1/3000. Predicted molecular weight: 11 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Tissue specificityDetected in testis, melanoma and bladder carcinoma.
Sequence similaritiesBelongs to the SPAN-X family.
Developmental stageDetected in round and elongating spermatids.
Cellular localizationCytoplasm. Nucleus. Associated with nuclear craters.
- Information by UniProt
- Cancer-testis-associated protein CTp11 antibody
- Cancer/testis antigen 11.3 antibody
- Cancer/testis-associated protein CTp11 antibody
All lanes : Anti-SPANX antibody (ab47252) at 1/1000 dilution
Lane 1 : Nuclear extract from VWM105 cells
Lane 2 : purified recombinant
SPANX-C fusion protein.
Lane 3 : purified
recombinant SPANX-B, protein.
Lane 4 : purified
recombinant SPANX-C, protein.
Lane 5 : purified
recombinant SPANX-N, protein.
Lanes 1-2 : IRDye™800 conjugated Gt-a-
Rabbit IgG [H&L] MX (611-132-122)
Predicted band size: 11 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa, 70 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab47252 has not yet been referenced specifically in any publications.