Product nameAnti-SPANX antibody
See all SPANX primary antibodies
DescriptionRabbit polyclonal to SPANX
SpecificityThe immunogen sequence is present within SPANX-A, SPANX-B, SPANX-C, SPANX-D and SPANX-N, but not present within SPANX-E.
Tested applicationsSuitable for: ELISA, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla
- Nuclear extract from VWM105 cells
General notesDilute only prior to immediate use.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab47252 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IF (microscopy): Use at an assay dependent dilution.
WB: 1/500 - 1/3000. Predicted molecular weight: 11 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Tissue specificityDetected in testis, melanoma and bladder carcinoma.
Sequence similaritiesBelongs to the SPAN-X family.
Developmental stageDetected in round and elongating spermatids.
Cellular localizationCytoplasm. Nucleus. Associated with nuclear craters.
- Information by UniProt
- Cancer-testis-associated protein CTp11 antibody
- Cancer/testis antigen 11.3 antibody
- Cancer/testis-associated protein CTp11 antibody
All lanes : Anti-SPANX antibody (ab47252) at 1/1000 dilution
Lane 1 : Nuclear extract from VWM105 cells
Lane 2 : purified recombinant
SPANX-C fusion protein.
Lane 3 : purified
recombinant SPANX-B, protein.
Lane 4 : purified
recombinant SPANX-C, protein.
Lane 5 : purified
recombinant SPANX-N, protein.
Lanes 1-2 : IRDye™800 conjugated Gt-a-
Rabbit IgG [H&L] MX (611-132-122)
Predicted band size: 11 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa, 70 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
ab47252 has not yet been referenced specifically in any publications.