For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Rabbit polyclonal to SPANX
The immunogen sequence is present within SPANX-A, SPANX-B, SPANX-C, SPANX-D and SPANX-N, but not present within SPANX-E.
HumanPredicted to work with:
, corresponding to internal sequence amino acids 31-47 of Human SPANX C
- Nuclear extract from VWM105 cells
Dilute only prior to immediate use.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/10000 - 1/50000.
IF (microscopy): Use at an assay dependent dilution.
WB: 1/500 - 1/3000. Predicted molecular weight: 11 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Detected in testis, melanoma and bladder carcinoma.
Belongs to the SPAN-X family.
Detected in round and elongating spermatids.
Cytoplasm. Nucleus. Associated with nuclear craters.
Information by UniProt
- Cancer-testis-associated protein CTp11 antibody
- Cancer/testis antigen 11.3 antibody
- Cancer/testis-associated protein CTp11 antibody
Western blot - Anti-SPANX antibody (ab47252)
All lanes :
Anti-SPANX antibody (ab47252) at 1/1000 dilutionLane 1 :
Nuclear extract from VWM105 cellsLane 2 :
SPANX-C fusion protein.Lane 3 :
recombinant SPANX-B, protein.Lane 4 :
recombinant SPANX-C, protein.Lane 5 :
recombinant SPANX-N, protein.SecondaryLanes 1-2 :
IRDye™800 conjugated Gt-a-
Rabbit IgG [H&L] MX (611-132-122)Predicted band size:
11 kDaObserved band size:
17 kDa (why is the actual band size different from the predicted?
)Additional bands at:
60 kDa, 70 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"