Product nameAnti-SPANX antibody
See all SPANX primary antibodies
DescriptionRabbit polyclonal to SPANX
SpecificityReacts with SPANX-C, SPANX-B and SPANX-N family members.
Tested applicationsSuitable for: IHC-P, WB, ELISA, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla
Full-length recombinant human SPANX-C protein.
- Nuclear extract from VWM105 cells, purified recombinant SPANX-B, SPANX-C and SPANX-N proteins.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
PurityProtein A purified
Purification notesab51493 was purified from monospecific antiserum by protein A chromatography followed by exhaustive dialysis against the buffer.
Our Abpromise guarantee covers the use of ab51493 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1.25 - 2.5 µg/ml.|
|WB||1/200 - 1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 11 kDa).|
|ELISA||1/1000 - 1/4000.|
|ICC/IF||Use at an assay dependent concentration.|
Tissue specificityDetected in testis, melanoma and bladder carcinoma.
Sequence similaritiesBelongs to the SPAN-X family.
Developmental stageDetected in round and elongating spermatids.
Cellular localizationCytoplasm. Nucleus. Associated with nuclear craters.
- Information by UniProt
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All lanes : Anti-SPANX antibody (ab51493) at 1/1000 dilution
Lane 1 : VWM105 cell nuclear extract
Lane 2 : purified recombinant SPANX-C fusion protein
Lane 3 : purified recombinant SPANX-B
Lane 4 : purified recombinant SPANX-C
Lane 5 : purified recombinant SPANX-N
Lane 1 : IRDye™800 conjugated Goat anti Rabbit
Lane 2 : IRDye™800 conjugated Goat anti Rabbit
Predicted band size: 11 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?
ab51493 at 2.5 ug/ml staining human sperm and spermatids at 60X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
ab51493 has not yet been referenced specifically in any publications.