Recombinant
RabMAb

Anti-SPANXD antibody [EPR8902] (ab169773)

Overview

  • Product name
    Anti-SPANXD antibody [EPR8902]
  • Description
    Rabbit monoclonal [EPR8902] to SPANXD
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human SPANXD aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9BXN6

  • Positive control
    • WB: Mouse testis lysate.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab169773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 11 kDa.

For unpurified use at 1/300.

  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Tissue specificity
      Detected in testis, sperm and a melanoma cell line.
    • Sequence similarities
      Belongs to the SPAN-X family.
    • Developmental stage
      Detected in round and elongating spermatids.
    • Cellular localization
      Cytoplasm. Nucleus. Associated with nuclear craters.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cancer/testis antigen 11.4 antibody
      • Cancer/testis antigen family 11 member 4 antibody
      • CT11.4 antibody
      • dJ171K16.1 antibody
      • Nuclear associated protein SPAN Xd antibody
      • Nuclear-associated protein SPAN-Xd antibody
      • SPAN Xd protein antibody
      • SPANX C antibody
      • SPANX D antibody
      • SPANX family member D antibody
      • SPANX-D antibody
      • SPANXD antibody
      • Sperm protein associated with the nucleus on the X chromosome D antibody
      • Sperm protein associated with the nucleus X chromosome family member D antibody
      • SPNXD_HUMAN antibody
      see all

    Images

    • Anti-SPANXD antibody [EPR8902] (ab169773) at 1/300 dilution (unpurified) + Mouse testis tissue lysate at 10 µg

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 11 kDa
      Observed band size: 13 kDa (why is the actual band size different from the predicted?)



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 1% BSA /TBST.

    • Anti-SPANXD antibody [EPR8902] (ab169773) at 1/2000 dilution (purified) + Mouse testis tissue lysate at 10 µg

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 11 kDa
      Observed band size: 13 kDa (why is the actual band size different from the predicted?)



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 1% BSA /TBST.

    References

    ab169773 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab169773.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up