RecombinantRabMAb

Recombinant Anti-SPANXD antibody [EPR8902] (ab169773)

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Western blot - Anti-SPANXD antibody [EPR8902] (ab169773)
  • Western blot - Anti-SPANXD antibody [EPR8902] (ab169773)
  • Anti-SPANXD antibody [EPR8902] (ab169773)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8902] to SPANXD
  • Suitable for: WB
  • Reacts with: Mouse

Overview

  • Product name

    Anti-SPANXD antibody [EPR8902]
    See all SPANXD primary antibodies

  • Description

    Rabbit monoclonal [EPR8902] to SPANXD

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Human

  • Immunogen

    Synthetic peptide within Human SPANXD aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9BXN6

  • Positive control

    • WB: Mouse testis lysate.

  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab169773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 11 kDa.

For unpurified use at 1/300.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 11 kDa.

For unpurified use at 1/300.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

    • Target

    • Tissue specificity

      Detected in testis, sperm and a melanoma cell line.

    • Sequence similarities

      Belongs to the SPAN-X family.

    • Developmental stage

      Detected in round and elongating spermatids.

    • Cellular localization

      Cytoplasm. Nucleus. Associated with nuclear craters.
    • Information by UniProt

    • Database links

      • Alternative names

        • Cancer/testis antigen 11.4 antibody
        • Cancer/testis antigen family 11 member 4 antibody
        • CT11.4 antibody
        • dJ171K16.1 antibody
        • Nuclear associated protein SPAN Xd antibody
        • Nuclear-associated protein SPAN-Xd antibody
        • SPAN Xd protein antibody
        • SPANX C antibody
        • SPANX D antibody
        • SPANX family member D antibody
        • SPANX-D antibody
        • SPANXD antibody
        • Sperm protein associated with the nucleus on the X chromosome D antibody
        • Sperm protein associated with the nucleus X chromosome family member D antibody
        • SPNXD_HUMAN antibody
        see all

      Images

      • Western blot - Anti-SPANXD antibody [EPR8902] (ab169773)
        Western blot - Anti-SPANXD antibody [EPR8902] (ab169773)
        Anti-SPANXD antibody [EPR8902] (ab169773) at 1/300 dilution (unpurified) + Mouse testis tissue lysate at 10 µg

        Secondary
        Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

        Predicted band size: 11 kDa
        Observed band size: 13 kDa why is the actual band size different from the predicted?



        Blocking buffer and concentration: 5% NFDM/TBST.

        Diluting buffer and concentration: 1% BSA /TBST.

      • Western blot - Anti-SPANXD antibody [EPR8902] (ab169773)
        Western blot - Anti-SPANXD antibody [EPR8902] (ab169773)
        Anti-SPANXD antibody [EPR8902] (ab169773) at 1/2000 dilution (purified) + Mouse testis tissue lysate at 10 µg

        Secondary
        Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

        Predicted band size: 11 kDa
        Observed band size: 13 kDa why is the actual band size different from the predicted?



        Blocking buffer and concentration: 5% NFDM/TBST.

        Diluting buffer and concentration: 1% BSA /TBST.

      • Anti-SPANXD antibody [EPR8902] (ab169773)
        Anti-SPANXD antibody [EPR8902] (ab169773)

      References (0)

      Publishing research using ab169773? Please let us know so that we can cite the reference in this datasheet.

      ab169773 has not yet been referenced specifically in any publications.

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