Anti-SPANXD antibody [EPR8902] (ab169773)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-SPANXD antibody [EPR8902]
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DescriptionRabbit monoclonal [EPR8902] to SPANXD
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivityReacts with: Mouse, Human
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Immunogen
Synthetic peptide within Human SPANXD aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: Q9BXN6 -
Positive control
- WB: Mouse testis lysate.
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR8902
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IsotypeIgG
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Research areas
Associated products
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab169773 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 11 kDa. For unpurified use at 1/300. |
Target
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Tissue specificityDetected in testis, sperm and a melanoma cell line.
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Sequence similaritiesBelongs to the SPAN-X family.
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Developmental stageDetected in round and elongating spermatids.
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Cellular localizationCytoplasm. Nucleus. Associated with nuclear craters.
- Information by UniProt
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Database links
- Entrez Gene: 64648 Human
- Omim: 300670 Human
- SwissProt: Q9BXN6 Human
- Unigene: 375036 Human
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Alternative names
- Cancer/testis antigen 11.4 antibody
- Cancer/testis antigen family 11 member 4 antibody
- CT11.4 antibody
see all
Images
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![Western blot - Anti-SPANXD antibody [EPR8902] (ab169773)](https://www.abcam.com/ps/products/169/ab169773/Images/ab169773-240881-ab169773upwb.jpg)
Western blot - Anti-SPANXD antibody [EPR8902] (ab169773)Anti-SPANXD antibody [EPR8902] (ab169773) at 1/300 dilution (unpurified) + Mouse testis tissue lysate at 10 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 11 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 1% BSA /TBST.
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![Western blot - Anti-SPANXD antibody [EPR8902] (ab169773)](https://www.abcam.com/ps/products/169/ab169773/Images/ab169773-240882-ab169773pwb.jpg)
Western blot - Anti-SPANXD antibody [EPR8902] (ab169773)Anti-SPANXD antibody [EPR8902] (ab169773) at 1/2000 dilution (purified) + Mouse testis tissue lysate at 10 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 11 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 1% BSA /TBST.
Protocols
Datasheets and documents
References
ab169773 has not yet been referenced specifically in any publications.
