Overview

  • Product name
  • Description
    Rabbit polyclonal to SPARC
  • Host species
    Rabbit
  • Specificity
    There were no cross reactivities obtained with human Osteopontin, and human Bone Sialoprotein.
  • Tested applications
    Suitable for: ELISA, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human).

Properties

Applications

Our Abpromise guarantee covers the use of ab14174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
IHC-P 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.

Target

  • Function
    Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
  • Sequence similarities
    Belongs to the SPARC family.
    Contains 1 EF-hand domain.
    Contains 1 follistatin-like domain.
    Contains 1 Kazal-like domain.
  • Developmental stage
    Expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix > basement membrane. In or around the basement membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AA517111 antibody
    • Basement membrane protein 40 antibody
    • Basement-membrane protein 40 antibody
    • BM 40 antibody
    • BM-40 antibody
    • BM40 antibody
    • Cysteine rich protein antibody
    • hm:zeh0062 antibody
    • MGC128090 antibody
    • OI17 antibody
    • ON antibody
    • Osteonectin antibody
    • Secreted acidic cystein rich glycoprotein antibody
    • Secreted protein acidic and cysteine rich antibody
    • Secreted protein acidic and rich in cysteine antibody
    • Secreted protein acidic cysteine rich (osteonectin) antibody
    • Secreted protein acidic cysteine rich antibody
    • SPARC antibody
    • SPRC antibody
    • SPRC_HUMAN antibody
    see all

Images

  • ab14174 at a 1/100 dilution staining SPARC in mouse endochondral bone by Immunohistochemistry (frozen sections) incubated for 16 hours at 4°C. PFA fixed. Permeabilized with 0.1% Triton. Blocked using 20% serum for 50 minutes at 19°C. Secondary used at 1/200 polyclonal Donkey anti-rabbit conjugated to Alexa Fluor 555 (red). Counterstain (blue) DAPI.

    See Abreview

  • ab14174 (1/1000) staining SPARC in human substanta nigra using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of the cytoplasm and extracellular matrix.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Immunohistochemical analysis of E14 and E16 mouse choroid plexus tissue, staining SPARC with ab14174. Sections were blocked with blocking agents and incubated with primary antibody (1/10000) overnight at 4°C. Staining was detected using DAB.

References

This product has been referenced in:
  • Kao SC  et al. A proteomics-based approach identifies secreted protein acidic and rich in cysteine as a prognostic biomarker in malignant pleural mesothelioma. Br J Cancer 114:524-31 (2016). Read more (PubMed: 26889976) »
  • Linhares MM  et al. Genetic and Immunohistochemical Expression of Integrins ITGAV, ITGA6, and ITGA3 As Prognostic Factor for Colorectal Cancer: Models for Global and Disease-Free Survival. PLoS One 10:e0144333 (2015). IHC . Read more (PubMed: 26674523) »
See all 8 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
IHC - Wholemount
Sample
Mouse Embryo (E4.5 Mouse Embryo)
Specification
E4.5 Mouse Embryo

Mr. Nicolas Porchet

Verified customer

Submitted Aug 29 2018

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample
Zebrafish Tissue sections (Skeleton)
Specification
Skeleton
Permeabilization
No
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Mar 12 2014

Question
Answer

DISCOUNT CODE: ***
Expiration date: May 30th, 2012

I am very pleased to hear you would like to accept our offer and test ab14174 in pig. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forpig and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Endochondral bone)
Specification
Endochondral bone
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
Serum as blocking agent for 50 minute(s) · Concentration: 20% · Temperature: 19°C

Abcam user community

Verified customer

Submitted Feb 17 2010

Question
Answer

Thank you for your enquiry. The immunogen for this antibody is recombinant full length Human protein. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for your enquiry. Ab19528 has been removed from our catalog. Ab14174 is a polyclonal antibody that was raised to the full length SPARC protein. Therefore, the antibody should recognize epitopes along the entire protein. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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