Key features and details
- Rabbit polyclonal to SPARC
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SPARC antibody
See all SPARC primary antibodies
DescriptionRabbit polyclonal to SPARC
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Cow, Pig, Orangutan
Recombinant fragment corresponding to Human SPARC aa 20-237. (BC004974).
QQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFDDGAEETEEEVVAE NPCQNHHCKHGKVCELDENNTPMCVCQDPTSCPAPIGEFEKVCSNDNKTF DSSCHFFATKCTLEGTKKGHKLHLDYIGPCKYIPPCLDSELTEFPLRMRD WLKNVLVTLYERDEDNNLLTEKQKLRVKKIHENEKRLEAGDHPVELLARD FEKNYNMYIFPVHWQFGQ
Database link: P09486
- Human fetal liver tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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FormLyophilized:Reconstitute in 200 µl of steriled distilled water.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab176975 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500.|
FunctionAppears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
Sequence similaritiesBelongs to the SPARC family.
Contains 1 EF-hand domain.
Contains 1 follistatin-like domain.
Contains 1 Kazal-like domain.
Developmental stageExpressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair.
Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane. In or around the basement membrane.
- Information by UniProt
- AA517111 antibody
- Basement membrane protein 40 antibody
- Basement-membrane protein 40 antibody
ab176975 has not yet been referenced specifically in any publications.