Overview

  • Product name

  • Description

    Rabbit polyclonal to SPARC
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SPARC aa 120-160 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    DSSCHFFATKCTLEGTKKGHKLHLDYIGPCKYIPPCLDSEL


    Database link: P09486

  • Positive control

    • IHC-P: Human lung carcinoma and colon carcinoma tissues; rat brain and mouse kidney tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab203284 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

When using a fluorescent probe the recommended dilution is 1/50 – 1/200.

Target

  • Function

    Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
  • Sequence similarities

    Belongs to the SPARC family.
    Contains 1 EF-hand domain.
    Contains 1 follistatin-like domain.
    Contains 1 Kazal-like domain.
  • Developmental stage

    Expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix > basement membrane. In or around the basement membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AA517111 antibody
    • Basement membrane protein 40 antibody
    • Basement-membrane protein 40 antibody
    • BM 40 antibody
    • BM-40 antibody
    • BM40 antibody
    • Cysteine rich protein antibody
    • hm:zeh0062 antibody
    • MGC128090 antibody
    • OI17 antibody
    • ON antibody
    • Osteonectin antibody
    • Secreted acidic cystein rich glycoprotein antibody
    • Secreted protein acidic and cysteine rich antibody
    • Secreted protein acidic and rich in cysteine antibody
    • Secreted protein acidic cysteine rich (osteonectin) antibody
    • Secreted protein acidic cysteine rich antibody
    • SPARC antibody
    • SPRC antibody
    • SPRC_HUMAN antibody
    see all

Images

  • Anti-SPARC antibody (ab203284) at 1/1000 dilution + A549 cell lysates

    Secondary
    Conjugated secondary antibody at 1/10000 dilution
  • Anti-SPARC antibody (ab203284) at 1/300 dilution + Mouse brain lysates

    Secondary
    Conjugated secondary antibody at 1/10000 dilution
  • Immunohistochemical analysis of formalin-fixed paraffin-embedded human lung carcinoma tissue labeling SPARC with ab203284 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded rat brain tissue labeling SPARC with ab203284 at 1/500 dilution, followed by Goat Anti-Rabbit IgG, Cy3 conjugated secondary antibody at 1/200 dilution and DAPI staining.

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded human colon carcinoma tissue labeling SPARC with ab203284 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded mouse kidney tissue labeling SPARC with ab203284 at 1/500 dilution, followed by conjugation to the secondary antibody and DAB staining.

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded human lung carcinoma tissue labeling SPARC with ab203284 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

References

ab203284 has not yet been referenced specifically in any publications.

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