• Product name

  • Description

    Rabbit polyclonal to SPARC
  • Host species

  • Tested applications

    Suitable for: IHC, ELISA, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide derived from the human SPARC protein.

  • Positive control

    • Mouse heart lysate



Our Abpromise guarantee covers the use of ab55847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC Use a concentration of 5 - 10 µg/ml.
ELISA Use a concentration of 1 - 5 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa). PubMed: 19509023


  • Function

    Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
  • Sequence similarities

    Belongs to the SPARC family.
    Contains 1 EF-hand domain.
    Contains 1 follistatin-like domain.
    Contains 1 Kazal-like domain.
  • Developmental stage

    Expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix > basement membrane. In or around the basement membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AA517111 antibody
    • Basement membrane protein 40 antibody
    • Basement-membrane protein 40 antibody
    • BM 40 antibody
    • BM-40 antibody
    • BM40 antibody
    • Cysteine rich protein antibody
    • hm:zeh0062 antibody
    • MGC128090 antibody
    • OI17 antibody
    • ON antibody
    • Osteonectin antibody
    • Secreted acidic cystein rich glycoprotein antibody
    • Secreted protein acidic and cysteine rich antibody
    • Secreted protein acidic and rich in cysteine antibody
    • Secreted protein acidic cysteine rich (osteonectin) antibody
    • Secreted protein acidic cysteine rich antibody
    • SPARC antibody
    • SPRC antibody
    • SPRC_HUMAN antibody
    see all


This product has been referenced in:

  • Farrokhi E  et al. Effect of Oxidized Low Density Lipoprotein on the Expression of Runx2 and SPARC Genes in Vascular Smooth Muscle Cells. Iran Biomed J 19:160-4 (2015). Read more (PubMed: 26025968) »
  • Liu Y  et al. Apratoxin a reversibly inhibits the secretory pathway by preventing cotranslational translocation. Mol Pharmacol 76:91-104 (2009). Read more (PubMed: 19403701) »
See all 3 Publications for this product

Customer reviews and Q&As


Thank you for your enquiry. The 75kDa band could be due to insufficient boiling of the sample, which resulted in the 75kDa dimer band. Since this image is confusing, I have removed it from the datasheet. Please rest assured that the product does detect the expected 35kDa band, as stated in the following publication. Kos K et al. Regulation of the fibrosis and angiogenesis promoter SPARC/osteonectin in human adipose tissue by weight change, leptin, insulin, and glucose. Diabetes 58:1780-8 (2009). WB; Human. PubMed: 19509023 http://diabetes.diabetesjournals.org/content/58/8/1780.long If you have any other questions, please do not hesitate to contact me.

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