Product nameAnti-SPARC antibody
See all SPARC primary antibodies
DescriptionRabbit polyclonal to SPARC
Tested applicationsSuitable for: IHC, ELISA, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide derived from the human SPARC protein.
- Mouse heart lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.08% Sodium azide
Concentration information loading...
Purification notesPurified by SAS precipitation.
Our Abpromise guarantee covers the use of ab55847 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC||Use a concentration of 5 - 10 µg/ml.|
|ELISA||Use a concentration of 1 - 5 µg/ml.|
|WB||Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa). PubMed: 19509023|
FunctionAppears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
Sequence similaritiesBelongs to the SPARC family.
Contains 1 EF-hand domain.
Contains 1 follistatin-like domain.
Contains 1 Kazal-like domain.
Developmental stageExpressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair.
Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane. In or around the basement membrane.
- Information by UniProt
- AA517111 antibody
- Basement membrane protein 40 antibody
- Basement-membrane protein 40 antibody
This product has been referenced in:
- Farrokhi E et al. Effect of Oxidized Low Density Lipoprotein on the Expression of Runx2 and SPARC Genes in Vascular Smooth Muscle Cells. Iran Biomed J 19:160-4 (2015). Read more (PubMed: 26025968) »
- Liu Y et al. Apratoxin a reversibly inhibits the secretory pathway by preventing cotranslational translocation. Mol Pharmacol 76:91-104 (2009). Read more (PubMed: 19403701) »