Overview

  • Product name

    Anti-SPARC antibody [RM0130-5F41]
    See all SPARC primary antibodies
  • Description

    Rat monoclonal [RM0130-5F41] to SPARC
  • Host species

    Rat
  • Tested applications

    Suitable for: ELISA, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant full length Mouse protein

Properties

Applications

Our Abpromise guarantee covers the use of ab86467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
IHC-Fr
  • Application notes
    Direct ELISA: 1/400 - 1/1000.
    IHC-Fr: 1/50 - 1/200.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
    • Sequence similarities

      Belongs to the SPARC family.
      Contains 1 EF-hand domain.
      Contains 1 follistatin-like domain.
      Contains 1 Kazal-like domain.
    • Developmental stage

      Expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair.
    • Cellular localization

      Secreted > extracellular space > extracellular matrix > basement membrane. In or around the basement membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • AA517111 antibody
      • Basement membrane protein 40 antibody
      • Basement-membrane protein 40 antibody
      • BM 40 antibody
      • BM-40 antibody
      • BM40 antibody
      • Cysteine rich protein antibody
      • hm:zeh0062 antibody
      • MGC128090 antibody
      • OI17 antibody
      • ON antibody
      • Osteonectin antibody
      • Secreted acidic cystein rich glycoprotein antibody
      • Secreted protein acidic and cysteine rich antibody
      • Secreted protein acidic and rich in cysteine antibody
      • Secreted protein acidic cysteine rich (osteonectin) antibody
      • Secreted protein acidic cysteine rich antibody
      • SPARC antibody
      • SPRC antibody
      • SPRC_HUMAN antibody
      see all

    References

    This product has been referenced in:

    • Puri C  et al. The RAB11A-Positive Compartment Is a Primary Platform for Autophagosome Assembly Mediated by WIPI2 Recognition of PI3P-RAB11A. Dev Cell 45:114-131.e8 (2018). Read more (PubMed: 29634932) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Answer

    Thank you for contacting us.

    The catalogue number is ab95462; https://www.abcam.com/Alkaline-Phosphatase-antibody-ab95462.html

    or

    ab113652; https://www.abcam.com/Alkaline-Phosphatase-antibody-SPM372-ab113652.html

    The compatible secondaries would be;

    https://www.abcam.com/goat-polyclonal-secondary-antibody-to-rabbit-igg-h-l-hrp-ab97051.html

    https://www.abcam.com/Donkey-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-HRP-ab97064.html

    https://www.abcam.com/goat-polyclonal-secondary-antibody-to-rabbit-igg-h-l-dylight-488-pre-adsorbed-ab96899.html

    https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG1-heavy-chain-FITC-ab97239.html

    https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG1-heavy-chain-HRP-ab97240.html

    https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG1-heavy-chain-Biotin-pre-adsorbed-ab98691.html

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Answer

    Thank you for contacting us.

    Please check the online datasheets ab64255 and ab64256 are already secondary antibodies.

    I suppose customer is looking for secondary antibody to ab86467. Please recommend them following; ab97057; https://www.abcam.com/goat-polyclonal-secondary-antibody-to-rat-igg-h-l-hrp-ab97057.html

    or

    ab98387; https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rat-IgG-H-L-DyLight-550-pre-adsorbed-ab98387.html

    or

    https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rat-IgG-H-L-DyLight-488-ab96887.html


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Answer

    Thank you for contacting us.

    We do not have Rat monoclonal anti BAP antibody, we however have mouse and rabbit monoclonal if customer is interested I can send the catalogue numbers.

    The Osteonectin antibody we have is ab86867; https://www.abcam.com/SPARC-antibody-RM0130-5F41-ab86467.html

    Abcam sells about 96,000 products; out of which more than 50,000 are antibodies. We believe in sharing of information so we publish all the data and even customer inquiries we get for our products. I can assure you that all the products are strict quality checked before publishing and we give 6 month guarantee which mean if the product don't work as expected we provide full refund.

    Regarding the quality these are of best quality however variations can be seen in the datasheets due to difference in samples being tested and lab conditions. Variation can also be significant in customer hands e.g. the room temperature her in UK is 25C which will be 30-40 in UAE so there are lot of factor at which results of antibodies depends. If you receive similar questions please provide reassurance to customer that if the product do not work we will refund your money.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

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