Overview

  • Product name

  • Description

    Rabbit polyclonal to SPARCL1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, between amino acids 215-244 from the Central region of Human SPARCL1 (NP_001121782.1, NP_004675.3).

  • Positive control

    • NCI-H460 cell line lysate, Human brain tissue

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab107533 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab107533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 75 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Tissue specificity

    Highly expressed in lymph node, brain, heart, lung, skeletal muscle, ovary, small intestine, and colon, with lower levels in placenta, pancreas, testis, spleen, and thymus, and no expression in kidney, liver, and peripheral blood leukocytes.
  • Sequence similarities

    Belongs to the SPARC family.
    Contains 1 EF-hand domain.
    Contains 1 follistatin-like domain.
    Contains 1 Kazal-like domain.
  • Post-translational
    modifications

    N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. Thr-398 is the preferred site to Thr-399.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • Ecm2 antibody
    • Extracellular matrix protein 2 antibody
    • Hevin antibody
    • High endothelial venule protein antibody
    • MAST 9 antibody
    • mast9 antibody
    • Matrix glycoprotein Sc1 antibody
    • PIG33 antibody
    • proliferation inducing protein 33 antibody
    • RAGS1 antigen antibody
    • SC1 antibody
    • SPARC like 1 antibody
    • SPARC-like protein 1 antibody
    • Sparcl1 antibody
    • SPRL1_HUMAN antibody
    see all

Images

  • Anti-SPARCL1 antibody (ab107533) at 1/100 dilution + NCI-H460 cell line lysate at 35 µg

    Predicted band size: 75 kDa

  • Immunohistochemistry analysis in formalin fixed and paraffin embedded Human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. ab107533 at a dilution of 1/50.

References

This product has been referenced in:

  • Zhao SJ  et al. SPARCL1 suppresses osteosarcoma metastasis and recruits macrophages by activation of canonical WNT/ß-catenin signaling through stabilization of the WNT-receptor complex. Oncogene 37:1049-1061 (2018). Read more (PubMed: 29084211) »
  • Han W  et al. Prognostic value of SPARCL1 in patients with colorectal cancer. Oncol Lett 15:1429-1434 (2018). Read more (PubMed: 29434834) »
See all 3 Publications for this product

Customer reviews and Q&As

Answer

Thank you for your inquiry.

I am sorry to confirm that we do not expect a cross reactivity of ab107533 with rat.

The immunogen only shows a 52% homology and we only expect a cross reactivity form 85% homology.

I am sorry that I did not have a positive answer on this occasion and hope this information is nevertheless helpful.

Read More

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