Key features and details
- Mouse monoclonal [Sp 3G11/1] to Spastin
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG2a
Product nameAnti-Spastin antibody [Sp 3G11/1]
See all Spastin primary antibodies
DescriptionMouse monoclonal [Sp 3G11/1] to Spastin
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Rat, Human
Recombinant full length protein (Human).
- WB: Total HeLa extract or rat brain synaptosome This antibody gave a positive result in IHC in the following FFPE tissue: Human lung adenocarcinoma.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A/G purified
Clone numberSp 3G11/1
Our Abpromise guarantee covers the use of ab31850 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Detects a band of approximately 52 kDa. There are two splice isoforms of spastin, one without exon4 and two alternative ATG start sites, which may determine the localisation of the translate protein. 52kDa is the major band.|
|IHC-P||Use at an assay dependent concentration.|
RelevanceSpastin is thought have a role in microtubule dynamics through its function as a microtubule severing protein. It is localised to the centrosome of neuronal cells but is not found in glial cells. Mutation in the ATPase binding domain of spastin causes hereditary spastic paraplegias (HSP), a large group of clinically similar disorders. Mutant forms of spastin are generally found throughout the cytoplasm rather then within the nucleus.
Cellular localizationCytoplasm, Cytoskeleton, Endoplasmic reticulum, Endosome, Membrane, Microtubule, Nucleus
- ADPSP antibody
- FSP 2 antibody
- FSP2 antibody
Anti-Spastin antibody [Sp 3G11/1] (ab31850) at 1/500 dilution + rat brain synaptosome
Observed band size: 52 kDa why is the actual band size different from the predicted?
There are two splice isoforms of spastin (one without exon4) and two alternative ATG start sites, which may determine the localisation of the translate protein. 52kDa is the major band.
IHC image of Spastin staining in Human lung adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab31850, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab31850 has been referenced in 2 publications.
- Daftary GS et al. A Novel Role for the AAA ATPase Spastin as a HOXA10 Transcriptional Corepressor in Ishikawa Endometrial Cells. Mol Endocrinol 25:1539-49 (2011). WB, ICC/IF, IHC-P, ChIP ; Human . PubMed: 21757506
- Salinas S et al. Human spastin has multiple microtubule-related functions. J Neurochem 95:1411-20 (2005). PubMed: 16219033