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We recently ordered two antibodies from you.
anti-spastin antibody, cat# ab31850
Anti-FIGN antibody, cat# ab122238
We have been testing both antibodies by WB and IF. By both WB and IF, both antibodies do not work. Is it possible to test a replacement or get a refund.
Asked on Jun 11 2012
Thank you for contacting us. I am sorry that these antibodies are not working in WB and IF. We will be happy to refund or replace the antibodies if they are being used in a tested species and application, and have been purchased in the past six months. In order to help our QC process, could you please provide some additional information?
1) What was your original order or PO number?
2) Could you please provide a description of the problem (no signal, high background, incorrect staining, etc) or an image of your results?
3) What type of samples were you testing? Which species were they from, what tissue type or cell line, and how were they prepared? For example, what lysis buffer was used in WB, were the samples reduced and denatured, andhow much protein was loaded? For IF, how were the cells fixed and permeabilized?
4) What were the dilutions and incubation times for the block, primary, and secondary antibodies? What blocking agent and secondary antibody were used?
I will be happy to assist you further once I have this additional information.
Answered on Jun 11 2012