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Thank you again for your reply. Below are the answers to your questions.
Please see attached blot image.
GPSM2 antibody – It would be great if we could get a replacement to try.
We have tried fixation in both Methanol and PFA as well and are unable to detect any protein in any conditions by IHC-P. As well, we have done multiple ECL exposure times and tested additional secondary antibody concentrations with no luck. Do you have any other suggestions?
Asked on Jul 23 2012
Thank you for your response.
I would expect ab31850 to give clearer results in WB, even once the many isoforms are taken into consideration. I would be happy to offer you a free of charge replacement, credit, or refund for this antibody as well. Please let me know which antibodies you would like to receive as replacements and I will be happy to process your request.
For the FIGN antibody ab122238, I would recommend ensuring that you are performing heat mediated antigen retrieval with Citrate buffer, pH 6. In order to increase the signal, it could help to lengthen your antigen retrieval time. Can you please let me know what type of tissue sections you were testing?
Answered on Jul 23 2012