• Product name

    Anti-Spastin antibody [Sp 6C6]
    See all Spastin primary antibodies
  • Description

    Mouse monoclonal [Sp 6C6] to Spastin
  • Host species

  • Tested applications

    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein (Human)

  • Positive control

    • Total HeLa extract or rat brain.



Our Abpromise guarantee covers the use of ab77144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 52 kDa (predicted molecular weight: 68 kDa).

There are two splice isoforms of spastin (one without exon4) and two alternative ATG start sites, which may determine the localisation of the translate protein. 52kDa is the major band.

ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.


  • Relevance

    Spastin is thought have a role in microtubule dynamics through its function as a microtubule severing protein. It is localised to the centrosome of neuronal cells but is not found in glial cells. Mutation in the ATPase binding domain of spastin causes hereditary spastic paraplegias (HSP), a large group of clinically similar disorders. Mutant forms of spastin are generally found throughout the cytoplasm rather then within the nucleus.
  • Cellular localization

    Cytoplasm, Cytoskeleton, Endoplasmic reticulum, Endosome, Membrane, Microtubule, Nucleus
  • Database links

  • Alternative names

    • ADPSP antibody
    • FSP 2 antibody
    • FSP2 antibody
    • KIAA1083 antibody
    • Spast antibody
    • spastic paraplegia 4 (autosomal dominant; spastin) antibody
    • Spastic paraplegia 4 antibody
    • spastin antibody
    • SPG 4 antibody
    • SPG4 antibody
    • SPG4 gene antibody
    see all


  • All lanes : Anti-Spastin antibody [Sp 6C6] (ab77144) at 1/1500 dilution

    All lanes : Human induced pluripotent stem cell derived neuron whole cell lysate

    Lysates/proteins at 20 µg per lane.

    All lanes : HRP-conjugated goat anti-mouse IgG polyclonal at 1/10 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 68 kDa
    Observed band size: 54,58,62,68 kDa
    why is the actual band size different from the predicted?

    Exposure time: 12 minutes

    9% SDS-PAGE.

    See Abreview


This product has been referenced in:

  • Havlicek S  et al. Gene dosage-dependent rescue of HSP neurite defects in SPG4 patients' neurons. Hum Mol Genet 23:2527-41 (2014). WB ; Human . Read more (PubMed: 24381312) »
  • Abrahamsen G  et al. A patient-derived stem cell model of hereditary spastic paraplegia with SPAST mutations. Dis Model Mech 6:489-502 (2013). WB ; Human . Read more (PubMed: 23264559) »
See all 3 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (9% SDS-PAGE)
Human Cell lysate - whole cell (human induced pluripotent stem cell derived neuron)
human induced pluripotent stem cell derived neuron
Blocking step
Blotting Grade blocker (BioRad) as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jun 23 2014


Thank you for having inquired about the epitope of ab77144 .

I am very sorry for the long delay of my answer - i have been in contact with the laboratory as well to see whether there was any additional information. Unfortunately, we have no information on the epitope. We have not performed any epitope mapping and I could not see a publication addressing the epitope neither.

I am sorry for not being able to help more on this occasion ( and that it took so long to get back to you). Please do however not hesitate to contact us again, should you have any other question.

I wish you good success with your research!

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First of all I would like to apologise for the delay in getting back to you. I really appreciate your patience and comprehension.

I contacted my lab colleagues and unfortunately there isn’t any information available about the epitope recognised by these antibodies, as they have not been mapped.

Please let me know if there is anything else I can help you with.

Read More

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