Product nameAnti-Spastin antibody [Sp 6C6]
See all Spastin primary antibodies
DescriptionMouse monoclonal [Sp 6C6] to Spastin
Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant full length protein (Human)
- Total HeLa extract or rat brain.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A/G purified
Clone numberSp 6C6
Our Abpromise guarantee covers the use of ab77144 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Detects a band of approximately 52 kDa (predicted molecular weight: 68 kDa).
There are two splice isoforms of spastin (one without exon4) and two alternative ATG start sites, which may determine the localisation of the translate protein. 52kDa is the major band.
|ELISA||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
RelevanceSpastin is thought have a role in microtubule dynamics through its function as a microtubule severing protein. It is localised to the centrosome of neuronal cells but is not found in glial cells. Mutation in the ATPase binding domain of spastin causes hereditary spastic paraplegias (HSP), a large group of clinically similar disorders. Mutant forms of spastin are generally found throughout the cytoplasm rather then within the nucleus.
Cellular localizationCytoplasm, Cytoskeleton, Endoplasmic reticulum, Endosome, Membrane, Microtubule, Nucleus
- ADPSP antibody
- FSP 2 antibody
- FSP2 antibody
All lanes : Anti-Spastin antibody [Sp 6C6] (ab77144) at 1/1500 dilution
All lanes : Human induced pluripotent stem cell derived neuron whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : HRP-conjugated goat anti-mouse IgG polyclonal at 1/10 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 54,58,62,68 kDa why is the actual band size different from the predicted?
Exposure time: 12 minutes
This product has been referenced in:
- Havlicek S et al. Gene dosage-dependent rescue of HSP neurite defects in SPG4 patients' neurons. Hum Mol Genet 23:2527-41 (2014). WB ; Human . Read more (PubMed: 24381312) »
- Abrahamsen G et al. A patient-derived stem cell model of hereditary spastic paraplegia with SPAST mutations. Dis Model Mech 6:489-502 (2013). WB ; Human . Read more (PubMed: 23264559) »