Product nameAnti-Spata18 antibody [EPR13704]
See all Spata18 primary antibodies
DescriptionRabbit monoclonal [EPR13704] to Spata18
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human Spata18 aa 100-250. The exact sequence is proprietary.
Database link: Q8TC71
- HepG2, HeLa, LnCaP and HT1080 cell lysates; Human kidney tissue; HepG2 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab180154 in the following tested applications.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/1000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).|
FunctionPotential role in spermatogenesis, especially in cell differentiation from late elongate spematids to mature spermatozoa.
- Information by UniProt
- 1700067I02Rik antibody
- FLJ32906 antibody
- MGC93900 antibody
All lanes : Anti-Spata18 antibody [EPR13704] (ab180154) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : LnCaP cell lysate
Lane 4 : HT1080 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 61 kDa
Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling Spata18 with ab180154 at 1/500 dilution, followed by Goat anti rabbit IgG (Dylight 555) at 1/250 dilution. Counter stained with Dapi.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Spata18 with ab180154 at 1/100 dilution, followed by prediluted Goat anti rabbit IgG (HRP). Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ab180154 has not yet been referenced specifically in any publications.