Product nameAnti-SPATA5L1 antibody
DescriptionRabbit polyclonal to SPATA5L1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
- Human pancreas tissue; RT4, U251 MG and Human tonsil tissue lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab126354 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/250 - 1/500. Predicted molecular weight: 81 kDa.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Sequence similaritiesBelongs to the AAA ATPase family. AFG2 subfamily.
- Information by UniProt
- FLJ12286 antibody
- MGC5347 antibody
- SPA5L_HUMAN antibody
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli. Recommended concentration of ab126354 1-4 µg/ml. Cells treated with PFA/Triton X-100.
All lanes : Anti-SPATA5L1 antibody (ab126354) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U251 MG cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Predicted band size: 81 kDa
ab126354 at a 1/50 dilution staining SPATA5L1 in paraffin embedded Human pancreas tissue by immunohistochemistry.
ab126354 has not yet been referenced specifically in any publications.