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Rabbit polyclonal to SPATA5L1
antigen sequence: TSCHCSFVSV SGADLFSPFV GDSEKVLSQI FRQARASTPA ILFLDEIDSI LGARSASKTG CDVQERVLSV LLNELDGVGL KTIERRGSKS SQQEFQEV, corresponding to amino acids 520-617 of Human SPATA5L1.
- Human pancreas tissue; RT4, U251 MG and Human tonsil tissue lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/250 - 1/500. Predicted molecular weight: 81 kDa.
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Belongs to the AAA ATPase family. AFG2 subfamily.
Information by UniProt
- FLJ12286 antibody
- MGC5347 antibody
- SPA5L_HUMAN antibody
Immunocytochemistry/ Immunofluorescence - Anti-SPATA5L1 antibody (ab126354)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli. Recommended concentration of ab126354 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-SPATA5L1 antibody (ab126354)
All lanes : Anti-SPATA5L1 antibody (ab126354) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U251 MG cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Predicted band size: 81 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPATA5L1 antibody (ab126354)
ab126354 at a 1/50 dilution staining SPATA5L1 in paraffin embedded Human pancreas tissue by immunohistochemistry.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"