Anti-SPC18 antibody (ab174794)
Key features and details
- Rabbit polyclonal to SPC18
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SPC18 antibody -
Description
Rabbit polyclonal to SPC18 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Orangutan -
Immunogen
Synthetic peptide within Human SPC18 aa 95-123 (internal sequence) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary. NM_014300.3, NP_055115.1.
Sequence:VHRVLKIHEKQNGHIKFLTKGDNNAVDDR
Database link: P67812 -
Positive control
- HepG2 cell line lysate; Human Lung tissue.
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General notes
Previously labelled as SC11A.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab174794 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
WB | 1/100 - 1/500. Predicted molecular weight: 21 kDa. |
Target
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Function
Component of the microsomal signal peptidase complex which removes signal peptides from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. -
Sequence similarities
Belongs to the peptidase S26B family. -
Cellular localization
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 282078 Cow
- Entrez Gene: 404004 Dog
- Entrez Gene: 23478 Human
- Entrez Gene: 56529 Mouse
- Entrez Gene: 100174516 Orangutan
- Entrez Gene: 65166 Rat
- SwissProt: P67810 Cow
- SwissProt: P67811 Dog
see all -
Alternative names
- 1810012E07Rik antibody
- Endopeptidase SP18 antibody
- Microsomal signal peptidase 18 kDa subunit antibody
see all
Images
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Anti-SPC18 antibody (ab174794) at 1/100 dilution + HepG2 cell line lysates at 35 µg
Predicted band size: 21 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPC18 antibody (ab174794)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung tissue labeling SPC18 with ab174794 at 5 μg/ml.
Protocols
Datasheets and documents
References (1)
ab174794 has been referenced in 1 publication.
- Shimura M et al. A novel liver metastasis-correlated protein of pancreatic neuroendocrine neoplasm (PanNEN) discovered by proteomic analysis. Oncotarget 9:24291-24303 (2018). PubMed: 29849941