Anti-SPC18 antibody (ab174794)

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Overview

  • Product name

    Anti-SPC18 antibody

  • Description

    Rabbit polyclonal to SPC18

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Orangutan

  • Immunogen

    Synthetic peptide within Human SPC18 aa 95-123 (internal sequence) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary. NM_014300.3, NP_055115.1.
    Sequence:

    VHRVLKIHEKQNGHIKFLTKGDNNAVDDR


    Database link: P67812

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • HepG2 cell line lysate; Human Lung tissue.

  • General notes

    Previously labelled as SC11A. 

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174794 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 21 kDa.

Target

Images

  • Western blot - Anti-SPC18 antibody (ab174794)
    Western blot - Anti-SPC18 antibody (ab174794)
    Anti-SPC18 antibody (ab174794) at 1/100 dilution + HepG2 cell line lysates at 35 µg

    Predicted band size: 21 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPC18 antibody (ab174794)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPC18 antibody (ab174794)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung tissue labeling SPC18 with ab174794 at 5 μg/ml.

References

This product has been referenced in:

  • Shimura M  et al. A novel liver metastasis-correlated protein of pancreatic neuroendocrine neoplasm (PanNEN) discovered by proteomic analysis. Oncotarget 9:24291-24303 (2018). Read more (PubMed: 29849941) »
See 1 Publication for this product

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