Anti-SPC18 antibody (ab174794)

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Western blot - Anti-SPC18 antibody (ab174794)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPC18 antibody (ab174794)

Key features and details

  • Rabbit polyclonal to SPC18
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-SPC18 antibody

  • Description

    Rabbit polyclonal to SPC18

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Orangutan

  • Immunogen

    Synthetic peptide within Human SPC18 aa 95-123 (internal sequence) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary. NM_014300.3, NP_055115.1.
    Sequence:

    VHRVLKIHEKQNGHIKFLTKGDNNAVDDR


    Database link: P67812
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • HepG2 cell line lysate; Human Lung tissue.

  • General notes

    Previously labelled as SC11A. 

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174794 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 21 kDa.

Target

Images

  • Western blot - Anti-SPC18 antibody (ab174794)
    Western blot - Anti-SPC18 antibody (ab174794)
    Anti-SPC18 antibody (ab174794) at 1/100 dilution + HepG2 cell line lysates at 35 µg

    Predicted band size: 21 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPC18 antibody (ab174794)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPC18 antibody (ab174794)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung tissue labeling SPC18 with ab174794 at 5 μg/ml.

References (1)

Publishing research using ab174794? Please let us know so that we can cite the reference in this datasheet.

ab174794 has been referenced in 1 publication.

  • Shimura M  et al. A novel liver metastasis-correlated protein of pancreatic neuroendocrine neoplasm (PanNEN) discovered by proteomic analysis. Oncotarget 9:24291-24303 (2018). PubMed: 29849941

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