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    spc24-antibody-epr11547b-ab157184.pdf

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Cell Biology Cell Cycle Cell Division Centromere
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RecombinantRabMAb

Recombinant Anti-SPC24 antibody [EPR11547(B)] (ab157184)

  • Datasheet
Reviews (1) Submit a question References (1)

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Abpromise

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Western blot - Anti-SPC24 antibody [EPR11547(B)] (ab157184)
  • Immunocytochemistry/ Immunofluorescence - Anti-SPC24 antibody [EPR11547(B)] (ab157184)
  • Anti-SPC24 antibody [EPR11547(B)] (ab157184)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11547(B)] to SPC24
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-SPC24 antibody [EPR11547(B)]
    See all SPC24 primary antibodies
  • Description

    Rabbit monoclonal [EPR11547(B)] to SPC24
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human SPC24 (UniProt: Q8NBT2).

  • Positive control

    • 293T, MOLT4, TF1 and HeLa cell lysates, Human thymus tissue lysate; 293T cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR11547(B)
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Centromere
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Chromosome Structure
    • Centromere

Associated products

  • Alternative Versions

    • Anti-SPC24 antibody [EPR11547(B)] - BSA and Azide free (ab249292)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • MOLT4 whole cell lysate (ab7912)

Applications

Our Abpromise guarantee covers the use of ab157184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 22 kDa.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function

      Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
    • Sequence similarities

      Belongs to the SPC24 family.
    • Cellular localization

      Nucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.
    • Target information above from: UniProt accession Q8NBT2 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 147841 Human
      • Omim: 609394 Human
      • SwissProt: Q8NBT2 Human
      • Unigene: 381225 Human
      • Alternative names

        • FLJ90806 antibody
        • hSpc24 antibody
        • Kinetochore protein Spc24 antibody
        • SPBC24 antibody
        • spc24 antibody
        • SPC24 NDC80 kinetochore complex component antibody
        • SPC24 NDC80 kinetochore complex component homolog antibody
        • SPC24, NDC80 kinetochore complex component, homolog (S. cerevisiae) antibody
        • SPC24_HUMAN antibody
        • Spindle pole body component 24 homolog (S. cerevisiae) antibody
        • Spindle pole body component 24 homolog antibody
        see all

      Images

      • Western blot - Anti-SPC24 antibody [EPR11547(B)] (ab157184)
        Western blot - Anti-SPC24 antibody [EPR11547(B)] (ab157184)
        All lanes : Anti-SPC24 antibody [EPR11547(B)] (ab157184) at 1/1000 dilution

        Lane 1 : 293T cell lysate
        Lane 2 : MOLT4 cell lysate
        Lane 3 : TF1 cell lysate
        Lane 4 : HeLa cell lysate
        Lane 5 : Human thymus tissue lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 22 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-SPC24 antibody [EPR11547(B)] (ab157184)
        Immunocytochemistry/ Immunofluorescence - Anti-SPC24 antibody [EPR11547(B)] (ab157184)
        Immunofluorescent analysis of 293T cells, labeling SPC24 with ab157184 at 1/50 dilution.
      • Anti-SPC24 antibody [EPR11547(B)] (ab157184)
        Anti-SPC24 antibody [EPR11547(B)] (ab157184)

      Protocols

      • Western blot protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (1)

      Publishing research using ab157184? Please let us know so that we can cite the reference in this datasheet.

      ab157184 has been referenced in 1 publication.

      • Thompson LL  et al. Reduced SKP1 Expression Induces Chromosome Instability through Aberrant Cyclin E1 Protein Turnover. Cancers (Basel) 12:N/A (2020). PubMed: 32106628

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Immunocytochemistry/ Immunofluorescence abreview for Anti-SPC24 antibody [EPR11547(B)]

      Poor
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (HeLa)
      Permeabilization
      Yes - 0.5% TritonX100 in PBS
      Specification
      HeLa
      Fixative
      Paraformaldehyde
      Read More

      Dr. Kirk Mcmanus

      Verified customer

      Submitted Jan 05 2016

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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