Recombinant Anti-SPC24 antibody [EPR11547(B)] (ab157184)
![Western blot - Anti-SPC24 antibody [EPR11547(B)] (ab157184)](https://www.abcam.com/ps/products/157/ab157184/Images/SPC24-Primary-antibodies-ab157184-1.jpg "Western blot - Anti-SPC24 antibody [EPR11547(B)] (ab157184)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11547(B)] to SPC24
- Suitable for: WB, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-SPC24 antibody [EPR11547(B)]
See all SPC24 primary antibodies -
Description
Rabbit monoclonal [EPR11547(B)] to SPC24 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide, corresponding to residues in Human SPC24 (UniProt: Q8NBT2).
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Positive control
- 293T, MOLT4, TF1 and HeLa cell lysates, Human thymus tissue lysate; 293T cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11547(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab157184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 22 kDa. | |
| ICC/IF | 1/50 - 1/100. |
Target
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Function
Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. -
Sequence similarities
Belongs to the SPC24 family. -
Cellular localization
Nucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore. - Information by UniProt
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Database links
- Entrez Gene: 147841 Human
- Omim: 609394 Human
- SwissProt: Q8NBT2 Human
- Unigene: 381225 Human
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Alternative names
- FLJ90806 antibody
- hSpc24 antibody
- Kinetochore protein Spc24 antibody
see all
Images
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Western blot - Anti-SPC24 antibody [EPR11547(B)] (ab157184)All lanes : Anti-SPC24 antibody [EPR11547(B)] (ab157184) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : MOLT4 cell lysate
Lane 3 : TF1 cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : Human thymus tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 22 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-SPC24 antibody [EPR11547(B)] (ab157184)](https://www.abcam.com/ps/products/157/ab157184/Images/SPC24-Primary-antibodies-ab157184-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SPC24 antibody [EPR11547(B)] (ab157184)Immunofluorescent analysis of 293T cells, labeling SPC24 with ab157184 at 1/50 dilution. -
![Anti-SPC24 antibody [EPR11547(B)] (ab157184)](https://www.abcam.com/ps/products/157/ab157184/Images/ab157184-3-benefits-of-recombinant-antibodies.png)
Anti-SPC24 antibody [EPR11547(B)] (ab157184)
Protocols
Datasheets and documents
References (1)
ab157184 has been referenced in 1 publication.
- Thompson LL et al. Reduced SKP1 Expression Induces Chromosome Instability through Aberrant Cyclin E1 Protein Turnover. Cancers (Basel) 12:N/A (2020). PubMed: 32106628
