Recombinant Anti-SPC24 antibody [EPR11547(B)] (ab157184)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-SPC24 antibody [EPR11547(B)]
See all SPC24 primary antibodies -
Description
Rabbit monoclonal [EPR11547(B)] to SPC24 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IPmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide, corresponding to residues in Human SPC24 (UniProt: Q8NBT2).
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Positive control
- 293T, MOLT4, TF1 and HeLa cell lysates, Human thymus tissue lysate; 293T cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11547(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab157184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 22 kDa. | |
| ICC/IF | 1/50 - 1/100. | |
| IP | 1/10 - 1/100. |
Target
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Function
Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. -
Sequence similarities
Belongs to the SPC24 family. -
Cellular localization
Nucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore. - Information by UniProt
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Database links
- Entrez Gene: 147841 Human
- Omim: 609394 Human
- SwissProt: Q8NBT2 Human
- Unigene: 381225 Human
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Alternative names
- FLJ90806 antibody
- hSpc24 antibody
- Kinetochore protein Spc24 antibody
see all
Images
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![Western blot - Anti-SPC24 antibody [EPR11547(B)] (ab157184)](https://www.abcam.com/ps/datasheet/images/157/ab157184/SPC24-Primary-antibodies-ab157184-1.jpg)
Western blot - Anti-SPC24 antibody [EPR11547(B)] (ab157184)All lanes : Anti-SPC24 antibody [EPR11547(B)] (ab157184) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : MOLT4 cell lysate
Lane 3 : TF1 cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : Human thymus tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 22 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-SPC24 antibody [EPR11547(B)] (ab157184)](https://www.abcam.com/ps/datasheet/images/157/ab157184/SPC24-Primary-antibodies-ab157184-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SPC24 antibody [EPR11547(B)] (ab157184)Immunofluorescent analysis of 293T cells, labeling SPC24 with ab157184 at 1/50 dilution.
Protocols
Datasheets and documents
References
ab157184 has not yet been referenced specifically in any publications.
