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Rabbit monoclonal [EPR11547(B)] to SPC24
IP more details Unsuitable for:
Flow Cyt or IHC-P
Synthetic peptide, corresponding to residues in Human SPC24 (UniProt: Q8NBT2).
293T, MOLT4, TF1 and HeLa cell lysates, Human thymus tissue lysate; 293T cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at -20ºC.
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Predicted molecular weight: 22 kDa.
1/50 - 1/100.
1/10 - 1/100.
Is unsuitable for Flow Cyt or IHC-P.
Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
Belongs to the SPC24 family.
Nucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.
Information by UniProt
Kinetochore protein Spc24 antibody
Western blot - Anti-SPC24 antibody [EPR11547(B)] (ab157184)
All lanes : Anti-SPC24 antibody [EPR11547(B)] (ab157184) at 1/1000 dilution Lane 1 : 293T cell lysate Lane 2 : MOLT4 cell lysate Lane 3 : TF1 cell lysate Lane 4 : HeLa cell lysate Lane 5 : Human thymus tissue lysate Lysates/proteins at 10 µg per lane. Secondary All lanes : Goat anti-rabbit HRP at 1/2000 dilution Predicted band size: 22 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SPC24 antibody [EPR11547(B)] (ab157184)
Immunofluorescent analysis of 293T cells, labeling SPC24 with ab157184 at 1/50 dilution.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"