Overview

  • Product name

  • Description

    Rabbit polyclonal to SPC25
  • Host species

    Rabbit
  • Specificity

    This antibody recognizes a protein of approximately 31kDa in HeLa cell lysate that is blocked by the addition of the immunizing peptide. This is larger than the expected size of 25kDa and we cannot explain why the detected protein is larger than expected. Several larger bands are detected which are not blocked at all by the addition of the immunizing peptide. SPC25 may be better detected in lysates of nocodazole-arrested HeLa cells.

  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human Spc25.

    Read Abcam's proprietary immunogen policy (Peptide available as ab23408.)

  • Positive control

    • This antibody gave a positive signal in the following lysates: NIH 3T3 (Mouse) Whole Cell Lysate (ab52956) Human pancreas tissue lysate - total protein (ab29816) Pancreas (Mouse) Tissue Lysate (ab55116) Ovary (Mouse) Tissue Lysate (ab35808) Ovary (Rat) Tissue Lysate (ab65552) HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) ICC/IF: HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab20679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 26 kDa).
ICC/IF 1/200.

Target

Images

  • All lanes : Anti-SPC25 antibody (ab20679) at 1 µg/ml

    Lane 1 : NIH 3T3 (Mouse) Whole Cell Lysate (ab52956)
    Lane 2 : Human ovary tissue lysate - total protein (ab30222)
    Lane 3 : Human pancreas tissue lysate - total protein (ab29816)
    Lane 4 : Pancreas (Mouse) Tissue Lysate (ab55116)
    Lane 5 : Ovary (Mouse) Tissue Lysate (ab35808)
    Lane 6 : Ovary (Rat) Tissue Lysate (ab65552)
    Lane 7 : Pancreas (Rat) Tissue Lysate (ab4036)
    Lane 8 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252)

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 26 kDa

  • ab20679 (1/200) staining SPC25 in assynchronous HeLa cells, in conjunction with a secondary goat anti-rabbit antibody conjugated to Cy3® (green). Cells were counterstained with DAPI to show the nucleus (red).

    See Abreview

References

This product has been referenced in:

  • Chen J  et al. SPC25 upregulation increases cancer stem cell properties in non-small cell lung adenocarcinoma cells and independently predicts poor survival. Biomed Pharmacother 100:233-239 (2018). WB . Read more (PubMed: 29432994) »
  • Villegas E  et al. Plk2 regulates mitotic spindle orientation and mammary gland development. Development 141:1562-71 (2014). WB . Read more (PubMed: 24598160) »
See all 3 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Formaldehyde
Permeabilization
Yes - 0.4% Triton-X
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C

Ms. Chelsea Backer

Verified customer

Submitted Apr 14 2009

Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
75 µg
Specification
HeLa
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Apr 13 2007

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Formaldehyde
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Feb 16 2007

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde

Dr. Kirk Mcmanus

Verified customer

Submitted Nov 02 2006

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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