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    spca2-antibody-n-terminal-ab176279.pdf

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Signal Transduction Metabolism Plasma Membrane ATPases
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Anti-SPCA2 antibody - N-terminal (ab176279)

  • Datasheet
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Western blot - Anti-SPCA2 antibody - N-terminal (ab176279)
  • Immunocytochemistry/ Immunofluorescence - Anti-SPCA2 antibody - N-terminal (ab176279)

Key features and details

  • Rabbit polyclonal to SPCA2 - N-terminal
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-SPCA2 antibody - N-terminal
  • Description

    Rabbit polyclonal to SPCA2 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human SPCA2 (N terminal). Peptide corresponds to 19 amino acids near the N terminus.
    Database link: O75185

  • Positive control

    • 3T3 cells and cell lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • ATPases

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

Our Abpromise guarantee covers the use of ab176279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 103 kDa.

Target

  • Function

    This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium.
  • Sequence similarities

    Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession O75185 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9914 Human
    • Entrez Gene: 69047 Mouse
    • Omim: 613082 Human
    • SwissProt: O75185 Human
    • SwissProt: A7L9Z8 Mouse
    • Unigene: 6168 Human
    • Unigene: 46251 Mouse
    • Alternative names

      • AT2C2_HUMAN antibody
      • ATP2C2 antibody
      • ATPase 2C2 antibody
      • ATPase, Ca++ transporting, type 2C, member 2 antibody
      • Calcium-transporting ATPase type 2C member 2 antibody
      • DKFZp686H22230 antibody
      • hSPCA2 antibody
      • KIAA0703 antibody
      • PMR1 antibody
      • Secretory pathway Ca(2+)-ATPase 2 antibody
      • secretory pathway calcium ATPase 2 antibody
      see all

    Images

    • Western blot - Anti-SPCA2 antibody - N-terminal (ab176279)
      Western blot - Anti-SPCA2 antibody - N-terminal (ab176279)
      All lanes : Anti-SPCA2 antibody - N-terminal (ab176279) at 1 µg/ml

      Lane 1 : 3T3 cell lysate
      Lane 2 : 3T3 cell lysate with Blocking peptide

      Lysates/proteins at 15 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 103 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-SPCA2 antibody - N-terminal (ab176279)
      Immunocytochemistry/ Immunofluorescence - Anti-SPCA2 antibody - N-terminal (ab176279)

      Immunofluorescent analysis of 3T3 cells, labeling SPCA2 with ab176279 at 20 μg/ml.

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab176279? Please let us know so that we can cite the reference in this datasheet.

    ab176279 has not yet been referenced specifically in any publications.

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