Key features and details
- Rabbit polyclonal to SPCA2 - N-terminal
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-SPCA2 antibody - N-terminal
DescriptionRabbit polyclonal to SPCA2 - N-terminal
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human SPCA2 (N terminal). Peptide corresponds to 19 amino acids near the N terminus.
Database link: O75185
- 3T3 cells and cell lysate.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab176279 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 20 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 103 kDa.|
FunctionThis magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium.
Sequence similaritiesBelongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.
- Information by UniProt
- AT2C2_HUMAN antibody
- ATP2C2 antibody
- ATPase 2C2 antibody
All lanes : Anti-SPCA2 antibody - N-terminal (ab176279) at 1 µg/ml
Lane 1 : 3T3 cell lysate
Lane 2 : 3T3 cell lysate with Blocking peptide
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Predicted band size: 103 kDa
Immunofluorescent analysis of 3T3 cells, labeling SPCA2 with ab176279 at 20 μg/ml.
ab176279 has not yet been referenced specifically in any publications.