Anti-SPCA2 antibody - N-terminal (ab176279)
Key features and details
- Rabbit polyclonal to SPCA2 - N-terminal
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SPCA2 antibody - N-terminal -
Description
Rabbit polyclonal to SPCA2 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human SPCA2 (N terminal). Peptide corresponds to 19 amino acids near the N terminus.
Database link: O75185 -
Positive control
- 3T3 cells and cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab176279 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 20 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 103 kDa. |
Target
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Function
This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium. -
Sequence similarities
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 9914 Human
- Entrez Gene: 69047 Mouse
- Omim: 613082 Human
- SwissProt: O75185 Human
- SwissProt: A7L9Z8 Mouse
- Unigene: 6168 Human
- Unigene: 46251 Mouse
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Alternative names
- AT2C2_HUMAN antibody
- ATP2C2 antibody
- ATPase 2C2 antibody
see all
Images
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Western blot - Anti-SPCA2 antibody - N-terminal (ab176279)All lanes : Anti-SPCA2 antibody - N-terminal (ab176279) at 1 µg/ml
Lane 1 : 3T3 cell lysate
Lane 2 : 3T3 cell lysate with Blocking peptide
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Predicted band size: 103 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-SPCA2 antibody - N-terminal (ab176279)Immunofluorescent analysis of 3T3 cells, labeling SPCA2 with ab176279 at 20 μg/ml.
Protocols
Datasheets and documents
References (0)
ab176279 has not yet been referenced specifically in any publications.
