Key features and details
- Rabbit polyclonal to SPCS2/SPC25
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-SPCS2/SPC25 antibody
See all SPCS2/SPC25 primary antibodies
DescriptionRabbit polyclonal to SPCS2/SPC25
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
- WB: RT-4, U-251 MG, Human liver and Human tonsil IHC-P: Human testis tissue ICC/IF: human cell line U-2 OS
This product was previously labelled as SPCS2
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121395 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 0.2 µg/ml.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionComponent of the microsomal signal peptidase complex which removes signal peptides from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum.
Sequence similaritiesBelongs to the SPCS2 family.
Cellular localizationMicrosome membrane. Endoplasmic reticulum membrane.
- Information by UniProt
- Microsomal signal peptidase 25 kDa subunit antibody
- Signal peptidase complex subunit 2 antibody
- Signal peptidase complex subunit 2 homolog (S. cerevisiae) antibody
Immunocytochemistry/immunofluorescence analysis of human cell line U-2 OS labelling SPCS2/SPC25 with ab121395 at 2 µg/mL
All lanes : Anti-SPCS2/SPC25 antibody (ab121395) at 1/250 dilution
Lane 1 : RT-4
Lane 2 : U-251 MG
Lane 3 : Human plasma
Lane 4 : Human liver
Lane 5 : Human tonsil
Developed using the ECL technique.
ab121395, at a 1/50 dilution, staining SPCS2/SPC25 in paraffin embedded Human testis tissue by Immunohistochemistry.
ab121395 has not yet been referenced specifically in any publications.