Anti-SPCS2/SPC25 antibody (ab236972)
Key features and details
- Rabbit polyclonal to SPCS2/SPC25
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SPCS2/SPC25 antibody
See all SPCS2/SPC25 primary antibodies -
Description
Rabbit polyclonal to SPCS2/SPC25 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Dog, Cynomolgus monkey, Orangutan -
Immunogen
Recombinant fragment corresponding to Human SPCS2/SPC25 aa 1-100.
Database link: Q15005 -
Positive control
- IHC-P: Human colon cancer and testis tissues. ICC/IF: HeLa cells. WB: K562 whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236972 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/5000. Predicted molecular weight: 25 kDa.
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IHC-P |
1/20 - 1/200.
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ICC/IF |
1/50 - 1/200.
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Notes |
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WB
1/500 - 1/5000. Predicted molecular weight: 25 kDa. |
IHC-P
1/20 - 1/200. |
ICC/IF
1/50 - 1/200. |
Target
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Function
Component of the microsomal signal peptidase complex which removes signal peptides from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. -
Sequence similarities
Belongs to the SPCS2 family. -
Cellular localization
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 102116673 Cynomolgus monkey
- Entrez Gene: 404016 Dog
- Entrez Gene: 9789 Human
- Entrez Gene: 66624 Mouse
- Entrez Gene: 100172541 Orangutan
- SwissProt: Q4R512 Cynomolgus monkey
- SwissProt: Q28250 Dog
- SwissProt: Q15005 Human
see all -
Alternative names
- Microsomal signal peptidase 25 kDa subunit antibody
- Signal peptidase complex subunit 2 antibody
- Signal peptidase complex subunit 2 homolog (S. cerevisiae) antibody
see all
Images
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Anti-SPCS2/SPC25 antibody (ab236972) at 1/500 dilution + K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 25 kDa -
HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for SPCS2/SPC25 (green) using ab236972 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
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Paraffin-embedded human testis tissue stained for SPCS2/SPC25 using ab236972 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human colon cancer tissue stained for SPCS2/SPC25 using ab236972 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab236972 has been referenced in 1 publication.
- Cui F et al. Spindle pole body component 25 and platelet-derived growth factor mediate crosstalk between tumor-associated macrophages and prostate cancer cells. Front Immunol 13:907636 (2022). PubMed: 35967419